Construct: ORF TRCN0000472911
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001219.1_s317c1
- Derived from:
- ccsbBroadEn_02623
- DNA Barcode:
- CACCACTGCGCCACTGGTCAGTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BTN2A1 (11120)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472911
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11120 | BTN2A1 | butyrophilin subfamily 2 me... | NM_078476.4 | 100% | 100% | |
| 2 | human | 11120 | BTN2A1 | butyrophilin subfamily 2 me... | NM_001197234.3 | 98.6% | 97.9% | (many diffs) |
| 3 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_001197238.2 | 78% | 72.3% | (many diffs) |
| 4 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_006714955.3 | 76.1% | 70.3% | (many diffs) |
| 5 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_017010168.2 | 76.1% | 70.3% | (many diffs) |
| 6 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_017010169.1 | 76.1% | 70.3% | (many diffs) |
| 7 | human | 11120 | BTN2A1 | butyrophilin subfamily 2 me... | NM_007049.5 | 63.1% | 62.5% | (many diffs) |
| 8 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_001197240.2 | 58.6% | 51.5% | (many diffs) |
| 9 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_001197237.2 | 51.9% | 47.9% | (many diffs) |
| 10 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_006995.5 | 51.9% | 47.9% | (many diffs) |
| 11 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_005248797.3 | 51.9% | 47.9% | (many diffs) |
| 12 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_006714953.4 | 51.9% | 47.9% | (many diffs) |
| 13 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_011514228.2 | 51.9% | 47.9% | (many diffs) |
| 14 | human | 11120 | BTN2A1 | butyrophilin subfamily 2 me... | NM_001197233.2 | 51.5% | 50.9% | (many diffs) |
| 15 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_011514229.2 | 42% | 38.9% | (many diffs) |
| 16 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_017010166.2 | 42% | 38.9% | (many diffs) |
| 17 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_024446302.1 | 42% | 38.9% | (many diffs) |
| 18 | human | 54718 | BTN2A3P | butyrophilin subfamily 2 me... | NR_027795.1 | 36.4% | (many diffs) | |
| 19 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_181531.3 | 31.9% | 28.2% | (many diffs) |
| 20 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_005248798.4 | 31.9% | 28.2% | (many diffs) |
| 21 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_017010167.1 | 31.9% | 28.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1068
- ORF length:
- 1002
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga atcagctgct gccctgcact tctcccggcc agcctccctc ctcctcctcc 121 tcctcagcct gtgtgcactg gtctcagccc agtttattgt cgtggggccc actgatccca 181 tcttggccac ggttggagaa aacactacgt tacgctgcca tctgtcaccc gagaaaaatg 241 ctgaggacat ggaggtgcgg tggttccggt ctcagttctc ccccgcagtg tttgtgtata 301 aaggtggcag agagagaaca gaggagcaga tggaggagta ccgaggaaga accacctttg 361 tgagcaaaga catcagcagg ggcagcgtgg ccctggtcat acacaacatc acagcccagg 421 aaaacggcac ctaccgctgt tacttccaag aaggcaggtc ctacgatgag gccatcctgc 481 acctcgtagt ggcaggacta ggctctaagc ccctcatttc aatgaggggc catgaagacg 541 ggggcatccg gctggagtgc atatctagag ggtggtaccc aaagcccctc acagtgtgga 601 gggaccccta cggtggggtt gcgCCTGCCC TGAAAGAGGT CTCCATGCCT GATGCAGACG 661 GCCTCTTCAT GGTCACCACG GCTGTGATCA TCAGAGACAA GTCTGTGAGG AACATGTCCT 721 GCTCTATCAA CAACACCCTG CTCGGCCAGA AGAAAGAAAG TGTCATTTTT ATTCCAGAAT 781 CCTTTATGCC CAGTGTGTCT CCCTGTGCAG TGGCCCTGCC TATCATTGTG GTTATTCTGA 841 TGATACCCAT TGCCGTATGC ATCTATTGGA TCAACAAACT CCAAAAGGAA AAAAAGATTC 901 TGTCAGGGGA AAAGGAGTTT GAACGGGAAA CAAGAGAAAT TGCTCTAAAG GAACTGGAGA 961 AAGAACGTGT GCAAAAAGAG GAAGAACTTC AAGTAAAAGA GAAACTTCAA GAAGAATTGC 1021 GATGGAGAAG AACATTCTTA CATGCTGAGC TCCAATTCTT CTCAAACTGC CCAACTTTCT 1081 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1141 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1201 GTGGAAAGGA CGACACCACT GCGCCACTGG TCAGTCTACG CGTTAAGTCg acaatcaacc 1261 tctggattac aaaatttgtg aaagatt