Construct: ORF TRCN0000472995
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015583.1_s317c1
- Derived from:
- ccsbBroadEn_02508
- DNA Barcode:
- GTTAACTTATATGCGCCGGCCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PNMA2 (10687)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472995
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10687 | PNMA2 | PNMA family member 2 | NM_007257.6 | 100% | 100% | |
| 2 | human | 10687 | PNMA2 | PNMA family member 2 | XM_011544365.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1158
- ORF length:
- 1092
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gctggcactg ttagaggact ggtgcaggat aatgagtgtg gatgagcaga 121 agtcactgat ggttacgggg ataccggcgg actttgagga ggctgagatt caggaggtcc 181 ttcaggagac tttaaagtct ctgggcaggt atagactgct tggcaagata ttccggaagc 241 aggagaatgc caatgctgtc ttactagagc ttctggaaga tactgatgtc tcggccattc 301 ccagtgaggt ccagggaaag gggggtgtct ggaaggtgat ctttaagacc cctaatcagg 361 acactgagtt tcttgaaaga ttgaacctgt ttctagaaaa agaggggcag acggtctcgg 421 gtatgtttcg agccctgggg caggagggcg tgtctccagc cacagtgccc tgcatctcac 481 cagaattact ggcccatttg ttgggacagg caatggcaca tgcgcctcag cccctgctac 541 ccatgagata ccggaaactg cgagtattct cagggagtgc tgtcccagcc ccagaggaag 601 agtcctttga ggtctggttg gaacaggcca cggagatagt caaagagtgg ccagtaacag 661 aggcagaaaa gaaaaggtgg ctggcggaaa gcctgcgggg ccctgccctg gacctcatgc 721 acatagtgca ggcagacaac ccgtccatca gtgtagaaga gtgtttggag gcctttaagc 781 aagtgtttgg gagcctagag agccgcagga cagcccaggt gaggtatctg aagacctatc 841 aggaggaagg agagaaggtc TCAGCCTATG TGTTACGGCT AGAAACCCTG CTCCGGAGAG 901 CGGTGGAGAA ACGCGCCATC CCTCGGCGTA TTGCGGACCA GGTCCGCCTG GAGCAGGTCA 961 TGGCTGGGGC CACTCTTAAC CAGATGCTGT GGTGCCGGCT TAGGGAGCTG AAGGATCAGG 1021 GCCCGCCCCC CAGCTTCCTT GAGCTAATGA AGGTAATACG GGAAGAAGAG GAGGAAGAGG 1081 CCTCCTTTGA GAATGAGAGT ATCGAAGAGC CAGAGGAACG AGATGGCTAT GGCCGCTGGA 1141 ATCATGAGGG AGACGACTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1201 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1261 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGTTAACT TATATGCGCC 1321 GGCCTCAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt