Construct: ORF TRCN0000473002
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009779.1_s317c1
- Derived from:
- ccsbBroadEn_02604
- DNA Barcode:
- GCCTCATTTGTTGCACGCTACACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- B4GAT1 (11041)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473002
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11041 | B4GAT1 | beta-1,4-glucuronyltransfer... | NM_006876.3 | 100% | 100% | |
| 2 | mouse | 108902 | B4gat1 | beta-1,4-glucuronyltransfer... | NM_175383.2 | 88.6% | 96.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1311
- ORF length:
- 1245
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gatgtcctac gccatccggt gcgccttcta ccagctgctg ctggccgcgc 121 tcatgctggt ggcgatgctg cagctgctct acctgtcgct gctgtccgga ctgcacgggc 181 aggaggagca agaccaatat tttgagttct ttcccccgtc cccacggtcc gtggaccagg 241 tcaaggcgca gctccgcacc gcgctggcct ctggaggcgt cctggacgct agcggcgatt 301 accgcgtcta caggggcctg ctgaagacca ccatggaccc caacgatgtg atcctggcca 361 cgcacgccag cgtggacaac ctgctgcacc tgtcgggtct gctggagcgc tgggagggcc 421 cgctgtccgt gtcggtgttc gcggccacca aggaggaggc gcagctggcc acggtgctgg 481 cctacgcgct gagcagccac tgccccgaca tgcgcgccag ggtcgccatg cacctcgtgt 541 gcccctcgcg ttacgaggca gccgtgcccg acccccggga gccgggggag tttgccctgc 601 tgcggtcctg ccaggaggtc tttgacaagc tagccagggt ggcccagccc gggattaatt 661 atgcgctggg caccaatgtc tcctacccca ataacctgct gaggaatctg gctcgtgagg 721 gggccaacta tgccctggtg atcgatgtgg acatggtgcc cagcgagggg ctgtggagag 781 gcctgcggga aatgctggat cagagcaacc agtggggagg caccgcgctg gtggtgcctg 841 ccttcgaaat ccgaagagcc cgccgcatgc ccatgaacaa aaacgagctg gtgcagctct 901 accaggttgg cgaggtgcgg cccttctatt atgggttgtg caccccctgc caggcaccca 961 ccaactattc ccgctgggTC AACCTGCCGG AAGAGAGCTT GCTGCGGCCC GCCTACGTGG 1021 TACCTTGGCA GGACCCCTGG GAGCCATTCT ACGTGGCAGG AGGCAAGGTG CCCACCTTCG 1081 ACGAGCGCTT TCGGCAGTAC GGCTTCAACC GAATCAGCCA GGCCTGCGAG CTGCATGTGG 1141 CGGGGTTTGA TTTTGAGGTC CTGAACGAAG GTTTCTTGGT TCATAAGGGC TTCAAAGAAG 1201 CGTTGAAGTT CCATCCCCAA AAGGAGGCTG AAAATCAGCA CAATAAGATC CTATATCGCC 1261 AGTTCAAACA GGAGTTGAAG GCCAAGTACC CCAACTCTCC CCGACGCTGC TGCCCAACTT 1321 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1381 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1441 CTTGTGGAAA GGACGAGCCT CATTTGTTGC ACGCTACACG ACGCGTTAAG TCgacaatca 1501 acctctggat tacaaaattt gtgaaagatt