Construct: ORF TRCN0000473038
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016142.1_s317c1
- Derived from:
- ccsbBroadEn_11265
- DNA Barcode:
- ACAAGTTTCATTTCTCTACAAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AXIN2 (8313)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473038
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8313 | AXIN2 | axin 2 | NM_001363813.1 | 19% | 17% | (many diffs) |
| 2 | human | 8313 | AXIN2 | axin 2 | XM_017025193.1 | 19% | 17% | (many diffs) |
| 3 | human | 8313 | AXIN2 | axin 2 | NM_004655.4 | 17.6% | 15.7% | (many diffs) |
| 4 | human | 8313 | AXIN2 | axin 2 | XM_011525319.2 | 17.6% | 15.7% | (many diffs) |
| 5 | human | 8313 | AXIN2 | axin 2 | XM_011525320.1 | 17.6% | 15.7% | (many diffs) |
| 6 | human | 8313 | AXIN2 | axin 2 | XM_011525321.2 | 17.6% | 15.7% | (many diffs) |
| 7 | human | 8313 | AXIN2 | axin 2 | XM_017025192.1 | 17.6% | 15.7% | (many diffs) |
| 8 | mouse | 12006 | Axin2 | axin 2 | NM_015732.4 | 15.5% | 15.1% | (many diffs) |
| 9 | mouse | 12006 | Axin2 | axin 2 | XM_006532057.3 | 15.5% | 15.1% | (many diffs) |
| 10 | mouse | 12006 | Axin2 | axin 2 | XM_006532058.1 | 15.5% | 15.1% | (many diffs) |
| 11 | mouse | 12006 | Axin2 | axin 2 | XM_006532059.3 | 15.5% | 15.1% | (many diffs) |
| 12 | mouse | 12006 | Axin2 | axin 2 | XM_006532060.1 | 15.5% | 15.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 546
- ORF length:
- 480
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 TTGGCATGAG TAGCGCTATG TTGGTGACTT GCCTCCCGGA CCCCAGCAGC AGCTTCCGTG 121 AGGATGCCCC GCGGCCCCCA GTGCCAGGGG AAGAAGGGGA GACCCCACCG TGTCAGCCAG 181 GGGTGGGCAA GGGCCAGGTC ACCAAACCCA TGCCTGTCTC TTCCAACACC AGGCGGAACG 241 AAGATGGGTT GGGGGAGCCG GAGGGGCGGG CATCTCCGGA TTCCCCTCTG ACCCGGTGGA 301 CCAAGTCCTT ACACTCCTTA TTGGGCGATC AAGACGGTGC TTACCTGTTC CGAACTTTCC 361 TGGAGAGGGA GAAATGCGTG GATACCTTAG ACTTCTGGTT TGCCTGCAAT GGATTCAGGC 421 AGATGAACCT GAAGGATACC AAAACTTTAC GAGTAGCCAA TGACTTTTGT CATGACATTT 481 TGTTCTACTT ATACTGTAAA TTATGCATTA TAAAGAGTTC ATTTAAGGAA AATTACTTGG 541 TACAATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 601 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 661 CTTGGCTTTA TATATCTTGT GGAAAGGACG AACAAGTTTC ATTTCTCTAC AAAACACGCG 721 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt