Construct: ORF TRCN0000473055
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004219.1_s317c1
- Derived from:
- ccsbBroadEn_15909
- DNA Barcode:
- ACGACGTAGACCAGTCTTTTTAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RADIL (55698)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473055
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55698 | RADIL | Rap associating with DIL do... | NM_018059.5 | 32.8% | 29.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1179
- ORF length:
- 1113
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tcctagggtg gggccagtca ctgtggccag gatcccagcc tcctgtgtcc 121 acagggcagg ggactcaggg ctcaatctgc tggcccctgg gttcccacac cgttgggcgg 181 ggcggcctgg cgagggaggc agcttctcct gtggccatta cgggcatcct ctctcccctg 241 cagaggatgt tctggagtcc tacgaaaacc ccccacccat cgtcctgccc agcgacggct 301 tccaggtgga cttggaagcc aactgcctgg acgacagcat ctaccagcac ctgctctacg 361 tccgccactt tctgtggggt ctgcggagca gagccagccc cggcagccct ggcaggcctg 421 gcagtggggc ctcccagcca gtgtgccccg agggtatgca ccacgtggtc cttgacgggc 481 acctggaggc cccgagctgc cccctggctc ccagggaccc tggcccagca gcccgggaag 541 tggccccgga gcgtactctt cccttgaggg gggctccctg ggcacaggcc ccccctggaa 601 ggcaacccgg ccgtgggggc tcccaggctg gccccccgca cacggactcg tcctgcttgc 661 tcacgcctcc cagcactcca cttggccctg agcctgggga ccccgactgg ccagagtccg 721 gcggcccctg tggaaaagcg cTCCCAGAGA GGCAGAGGAA TGGACCCAGC GGCCTCCGGG 781 GTGCAGCTCC GGAAGGAGAC TCTGCAGCCC TTGCGGAGGA GTCCCCTCCA GCCCCGTCCA 841 GCCGCAGCTC CAGCACCGAG GACTTCTGCT ACGTCTTCAC GGTGGAGCTG GAACGAGGCC 901 CCTCCGGGCT GGGGATGGGC CTGATCGACG GGATGCACAC GCACCTGGGC GCCCCCGGGC 961 TCTACATCCA GACCCTGCTC CCGGGCAGCC CCGCAGCGGC CGACGGGCGC CTGTCGCTGG 1021 GGGACCGTAT CCTGGAGGTG AATGGCAGCA GCCTCCTGGG CCTTGGCTAC CTGAGAGCTG 1081 TGGACCTGAT CCGTCATGGC GGGAAGAAGA TGCGGTTCCT GGTCGCGAAG TCCGACGTGG 1141 AAACAGCCAA GAAGATCCAT TTCCGCACGC CCCCTCTCTA CCCAACTTTC TTGTACAAAG 1201 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1261 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1321 ACGAACGACG TAGACCAGTC TTTTTAAAAC GCGTTAAGTC gacaatcaac ctctggatta 1381 caaaatttgt gaaagatt