Construct: ORF TRCN0000473081
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002812.1_s317c1
- Derived from:
- ccsbBroadEn_06395
- DNA Barcode:
- AGTTTGTAGCAAGGACTCTTTGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOXC11 (3227)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473081
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3227 | HOXC11 | homeobox C11 | NM_014212.4 | 99.6% | 99.3% | 36T>G;143C>T;491A>N |
| 2 | mouse | 109663 | Hoxc11 | homeobox C11 | NM_001024842.1 | 93.5% | 97% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 978
- ORF length:
- 912
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt taactcggtc aacctgggca acttctgctc gccgtcgcgc aaggagaggg 121 gcgcagattt cggcgagcga gggagctgcg cctccaacct ctatctgccc agttgcactt 181 actacatgcc cgagttctcc acggtcttct ccttcctgcc ccaggccccc tctcgtcaga 241 tctcctatcc ctactcggcc caagtgcccc cggtccggga ggtctcctac ggcctggagc 301 catccggcaa gtggcaccat cggaacagct actcctcctg ctatgcggcg gccgacgagc 361 ttatgcaccg ggagtgcctg cctccttcca ccgtcaccga gatcctcatg aaaaacgaag 421 gctcctacgg cggccaccac caccccagcg ccccgcacgc aacccccgcc ggcttctact 481 cctcagtcaa caagaacagc gtcctgcctc aagccttcga ccgtttcttc gacaacgcct 541 actgcggtgg cggcgncccg cccgccgagc cccccTGCTC CGGCAAGGGC GAGGCCAAGG 601 GGGAGCCCGA GGCACCCCCG GCCTCGGGAC TGGCGTCCCG GGCTGAGGCG GGTGCCGAGG 661 CGGAGGCTGA GGAGGAGAAC ACAAATCCCA GCTCGTCCGG TTCAGCCCAC TCCGTGGCCA 721 AGGAGCCGGC CAAAGGAGCC GCCCCCAACG CCCCCCGCAC CCGCAAGAAG CGCTGCCCTT 781 ATTCGAAATT CCAGATCCGG GAACTGGAGC GAGAGTTTTT CTTCAACGTG TATATCAACA 841 AAGAGAAGCG GCTGCAGCTG TCCCGGATGC TGAACCTGAC GGACCGACAA GTGAAAATTT 901 GGTTTCAGAA CAGAAGGATG AAAGAAAAGA AACTGAGCAG AGACCGGCTG CAGTATTTCT 961 CGGGAAATCC TCTGCTGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1021 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1081 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGTTTGT AGCAAGGACT 1141 CTTTGACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt