Construct: ORF TRCN0000473106
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010714.1_s317c1
- Derived from:
- ccsbBroadEn_02133
- DNA Barcode:
- TTTCGCTGATCCTACAGGTTGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD83 (9308)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473106
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9308 | CD83 | CD83 molecule | NM_004233.4 | 100% | 100% | |
| 2 | human | 9308 | CD83 | CD83 molecule | NM_001040280.2 | 99.5% | 99.5% | 489_490insAAG |
| 3 | human | 9308 | CD83 | CD83 molecule | NM_001251901.1 | 71.2% | 71.2% | 0_1ins177 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 681
- ORF length:
- 615
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gcgcggcctc cagcttctgc tcctgagctg cgcctacagc ctggctcccg 121 cgacgccgga ggtgaaggtg gcttgctccg aagatgtgga cttgccctgc accgccccct 181 gggatccgca ggttccctac acggtctcct gggtcaagtt attggagggt ggtgaagaga 241 ggatggagac accccaggaa gaccaccTCA GGGGACAGCA CTATCATCAG AAGGGGCAAA 301 ATGGTTCTTT CGACGCCCCC AATGAAAGGC CCTATTCCCT GAAGATCCGA AACACTACCA 361 GCTGCAACTC GGGGACATAC AGGTGCACTC TGCAGGACCC GGATGGGCAG AGAAACCTAA 421 GTGGCAAGGT GATCTTGAGA GTGACAGGAT GCCCTGCACA GCGTAAAGAA GAGACTTTTA 481 AGAAATACAG AGCGGAGATT GTCCTGCTGC TGGCTCTGGT TATTTTCTAC TTAACACTCA 541 TCATTTTCAC TTGTAAGTTT GCACGGCTAC AGAGTATCTT CCCAGATTTT TCTAAAGCTG 601 GCATGGAACG AGCTTTTCTC CCAGTTACCT CCCCAAATAA GCATTTAGGG CTAGTGACTC 661 CTCACAAGAC AGAACTGGTA TGCCCAATTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 721 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 781 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTTC GCTGATCCTA 841 CAGGTTGATC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt