Construct: ORF TRCN0000473115
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010052.1_s317c1
- Derived from:
- ccsbBroadEn_12400
- DNA Barcode:
- TAGGTAGCTTAGGAGTGCACAAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEMA4G (57715)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473115
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57715 | SEMA4G | semaphorin 4G | NM_001203244.1 | 18% | 18% | 1_1725del |
| 2 | human | 57715 | SEMA4G | semaphorin 4G | XR_246101.3 | 8.7% | 1_3236del;3618_4345del | |
| 3 | human | 57715 | SEMA4G | semaphorin 4G | XR_945792.2 | 8.3% | 1_3469del;3851_4578del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 447
- ORF length:
- 381
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctcaatgtca ccaccctctg catggccctg tgtgctggat ggtcctgaaa 121 ccagacaaga cctctgccag ccaccTAAGC CCTGCGTACA TTCACATGCA CACATGGAAG 181 AATGTTTATC GGCTGGGCTG CAGTGCCCCC ACCCTCACCT TCTCCTGGTG CATTCTTGTT 241 TCATCCCTGC TTCTGGACTT GGGGTACCCT CCCAATTGCC ACATCCTATC TGGTCCTCTT 301 CCCCAGCCCC ATGTGGTGAC CTCTTTGTCA AGAGCTTGGG AACGGGCCAG CCTGGGGAGG 361 TAAGACTGCA TCACTCCCCT CCTCTCCCTT CCTGTGTGGC CCTTGTGAAT CAGCCTCCCC 421 ACTCTCCTTG GTCATTCTCA AGAGTATGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 481 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 541 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATAGGTAGC 601 TTAGGAGTGC ACAAGGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 661 aagatt