Construct: ORF TRCN0000473131
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013852.4_s317c1
- Derived from:
- ccsbBroadEn_06709
- DNA Barcode:
- GAACTGAATACACAAGCCCGCTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDK4 (5166)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473131
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5166 | PDK4 | pyruvate dehydrogenase kina... | NM_002612.4 | 99.9% | 100% | 1023A>C |
| 2 | mouse | 27273 | Pdk4 | pyruvate dehydrogenase kina... | NM_013743.2 | 86.5% | 92.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1299
- ORF length:
- 1233
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggcggcccgc ttcgtgctgc gcagcgctgg ctcgctcaac ggcgccggcc 121 tggtgccccg agaggtggag catttctcgc gctacagccc gtccccgctg tccatgaagc 181 agctactgga ctttggttca gaaaatgcat gtgaaagaac ttcttttgca tttttgcgac 241 aagaattgcc tgtgagactc gccaacattc tgaaggaaat tgatatcctc ccgacccaat 301 tagtaaatac ctcttcagtg caattggtta aaagctggta tatacagagc ctgatggatt 361 tggtggaatt ccatgagaaa agcccagatg accagaaagc attatcagac tttgtagata 421 cactcatcaa agttcgaaat agacaccata atgtagtccc tacaatggca caaggaatca 481 tagagtataa agatgcctgt acagttgacc cagtcaccaa tcaaaatctt caatatttct 541 tggatcgatt ttacatgaac cgtatttcta ctcggatgct gatgaaccag cacattctta 601 tatttagtga ctcacagaca ggaaacccaa gccacattgg aagcattgat cctaactgtg 661 atgtggtagc agtggtccaa gatgcctttg agtgttcaag gatgctctgt gatcagtatt 721 atttatcatc tccagaatta aagcttacac aagtgaatgg aaaatttcca gaccaaccaa 781 ttcacatcgt gtatgttcct tctcacctCC ATCATATGCT CTTTGAACTA TTTAAGAATG 841 CAATGCGGGC AACAGTTGAA CACCAGGAAA ATCAGCCTTC CCTTACACCA ATAGAGGTTA 901 TTGTTGTCTT GGGAAAAGAA GACCTTACCA TTAAGATTTC AGACAGAGGA GGTGGTGTTC 961 CCCTGAGAAT TATTGACCGC CTCTTTAGTT ATACATACTC CACTGCACCA ACGCCTGTGA 1021 TGGATAATTC CCGGAATGCT CCTTTGGCTG GTTTTGGTTA CGGCTTGCCA ATTTCTCGTC 1081 TGTATGCCAA GTACTTTCAA GGAGATCTGA ATCTCTACTC TTTATCAGGA TATGGAACAG 1141 ATGCTATCAT CTACTTAAAG GCTTTGTCTT CTGAGTCTAT AGAAAAACTT CCAGTTTTTA 1201 ACAAGTCAGC CTTCAAACAT TATCAGATGA GCTCTGAGGC TGATGACTGG TGTATCCCAA 1261 GCAGGGAACC AAAGAACCTG GCAAAAGAAG TGGCCATGTG CCCAACTTTC TTGTACAAAG 1321 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1381 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1441 ACGAGAACTG AATACACAAG CCCGCTCCAC GCGTTAAGTC gacaatcaac ctctggatta 1501 caaaatttgt gaaagatt