Construct: ORF TRCN0000473160
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007340.1_s317c1
- Derived from:
- ccsbBroadEn_13479
- DNA Barcode:
- TTAACAGCTGGCCGCCTCCTTGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CATSPERD (257062)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473160
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_011527892.1 | 85.6% | 85.5% | 372C>G;743_940del;1366C>G |
| 2 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_011527891.1 | 76.3% | 76.2% | (many diffs) |
| 3 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_017026566.2 | 54.8% | 54.8% | (many diffs) |
| 4 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_017026565.1 | 50.4% | 50.3% | (many diffs) |
| 5 | human | 257062 | CATSPERD | cation channel sperm associ... | NM_152784.4 | 49.9% | 49.8% | 1_1197del;1569C>G;2365C>G |
| 6 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_017026564.2 | 47.6% | 47.6% | (many diffs) |
| 7 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_011527886.3 | 47% | 47% | (many diffs) |
| 8 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_011527885.3 | 46.1% | 46% | (many diffs) |
| 9 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_011527887.3 | 41.1% | 41% | (many diffs) |
| 10 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_011527888.3 | 31.2% | 31% | (many diffs) |
| 11 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_011527889.3 | 23.6% | 23.6% | (many diffs) |
| 12 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_011527890.3 | 18.4% | 18.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1263
- ORF length:
- 1197
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta taccattgac atgcacagcc agctggaatt gactgcttcg ttgatacccc 121 agccaggcac atccctgatt cctctggtga tggtgagcaa cccccactcc ctggggttcc 181 aggccacctt ctacgagaac ggttacacat cagatgggaa caccaagtac aaactggata 241 ttttcctaaa acagcagcag cactggggca ggaccgactc caacttcact tccagtttaa 301 agaaagccac catgtctacc ttaactgtgg acatagcaaa caaggaaatt tcatgtgtgg 361 atatcaagcc actgtcgaca ctgatttcag ttggctgcga cctggataaa aagatcgtca 421 tccagaacaa agtttcggcc tgttccatgg gcatcctgga ccccttgacc ctgcaagaca 481 attacagctt catcatcgag aaggaattct acgaccccgg cttccagggg cagcagtcct 541 ccgaggacct gcacgtgttt tactcctacc agcagctggg ctgtcctctc ctcgtctact 601 atgacaccct atggaagccc gtggtggagc tgtggcgaaa agacagtttc caggaggtca 661 tcgacgccga gtatgtgtta ctggaggtga acgggcagtt ctcatactcc tattccctga 721 cggcccagtc ggccatgtgt accTCCCAGC CGCAGAACTG GACCACCATG ATAAAGGAAT 781 TCGGGGGGCC CTTCTTCTGG AACAGAGAGA ACTATGTGAG CTGCCACGAC CCCAACAACA 841 ATGCCCCTTT GAGGTGGCCA GACGTCCAGT ATCAGATCTT GGGCGGCCGG ACAGCAAACC 901 AGATCATTTT CGGCCACAAT GGCTTTTATG TCTTCTACAT TTCGATCGTG GATCCGTACT 961 ACAGCTACTG TCAACTGGAG ACCATCTTTA GCATCTACGT GTATGGAGCA TTCCCCGTGC 1021 AGCTGGTCTC TGCTGGAGTC GTCATCCTAC TGATCATCTC CAGCATCCTG GGGTCCGTTT 1081 GGCTGGCCTA CAAGACCCCC AAGCTGCTAC GCACAGCACG CGGCCGCAGG ATCAAGAAGT 1141 GTGCGACACA GCTGTGTAGG AGATGCAAGA CGGTCTGCCA GTTCAGGGCC TCAGCCACAG 1201 CCAGGGCAGG CACAGAGCCC CCGGGACGCC ACGGCACTCC TCACGGAGGC AGGTCTGACC 1261 ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1321 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1381 GGCTTTATAT ATCTTGTGGA AAGGACGATT AACAGCTGGC CGCCTCCTTG CAACGCGTTA 1441 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt