Construct: ORF TRCN0000473182
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001628.1_s317c1
- Derived from:
- ccsbBroadEn_12421
- DNA Barcode:
- CGCAACGGTACAAGAGTTCCCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MPP4 (58538)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473182
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58538 | MPP4 | membrane palmitoylated prot... | NM_033066.3 | 98.7% | 98.5% | (many diffs) |
| 2 | human | 58538 | MPP4 | membrane palmitoylated prot... | XM_017004620.1 | 96% | 95.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1959
- ORF length:
- 1890
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatacagtca gacaaaggag cagatccacc agacaagaag gacatgaagc 121 tttctacagc caccaatcca cagaatggcc tctcccagat cctgaggctt gtgctgcaag 181 agctgagtct gttctacagc agagatgtga atggagtgtg tctcttgtac gatctcctcc 241 actcgccgtg gcttcaggct ctgctaaaga tttatgactg cctccaggaa tttaaagaaa 301 agaaactagt tcctgccaca ccacatgcac aggtgttatc ctatgaggta gtggagttat 361 tacgtgaaac ccctacttcc cctgagatcc aagagctgag acaaatgctc caggctccac 421 acttcaaggc cttgctcagt gcccatgaca cgatagctca gaaagatttt gaaccccttc 481 tccctccact gccagacaat atccctgaga gtgaggaagc aatgaggatt gtttgtttag 541 tgaaaaacca acagcccctg ggagccacca tcaagcgcca cgagatgaca ggggacatct 601 tggtggccag gatcatccac ggtgggctgg cggagagaag tgggttgcta tatgctggag 661 acaaactggt agaagtgaat ggagtttcag ttgagggact ggaccctgaa caagtgatcc 721 atattctggc catgtctcga ggcacaatca tgttcaaggt ggttccagtc tctgaccctc 781 ctgtgaatag ccagcagatg gtgtacgtcc gtgccatgac tgagtactgg ccccaggagg 841 atcccgacat cccctgcatg gacgctggat tgcctttcca gaagggggac atcctccaga 901 ttgtggacca gaatgatgcc ctctggtggc aggcccgaaa aatctcagac cctgctacct 961 gcgctgggct tgtcccttct aaccaccttc tgaagaggaa gcaacgggaa ttctggtggt 1021 ctcagccgta ccagcctcac acctgcctca agtcaaccct atcaatttct atggaagaag 1081 aagatgacat gaagattgat gagaaatgtg tggaagcaga ggaactttca gaagacaagg 1141 aggagtttgt tggctacggt cagaagttct ttatagctgg cttccgccgc agcatgcgcc 1201 tttgtcgcag gaagtctcac ctcagcccgc tgcatgccag tgtgtgctgc accggcagct 1261 gctacagtgc agtgggtgcc ccttacgagg aggtggtgag gtaccagcga cgcccttcag 1321 acaagtaccg cctcatagtg ctcatgggac cctctggtgt tggagtaaat gagctcagaa 1381 gacaacttat tgaatttaat cccagccatt ttcaaagtgc tgtgccacac actactcgta 1441 ctaaaaagag ttacgaaatg aatgggcgtg agtatcacta tgtgtccaag gaaacatttg 1501 aaaaccTCAT ATATAGTCAC AGGATGCTGG AGTATGGTGA GTACAAAGGC CACCTGTATG 1561 GCACTAGTGT GGATGCTGTT CAAACAGTCC TTGTCGAAGG AAAGATCTGT GTCATGGACC 1621 TAGAGCCTCA GGATATTCAA GGGGTTCGAA CCCATGAACT GAAGCCCTAT GTCATATTTA 1681 TAAAGCCATC GAATATGAGG TGTATGAAAC AATCTCGGAA AAATGCCAAG ATTATTACTG 1741 ACTATTATGT GGACATGAAG TTCAAGGATG AAGACCTACA AGAGATGGAA AATTTAGCCC 1801 AAAGAATGGA AACTCAGTTT GGCCAATTTT TTGATCATGT GATTGTGAAT GACAGCTTGC 1861 ACGATGCATG TGCCCAGTTG TTGTCTGCCA TACAGAAGGC TCAGGAGGAG CCTCAGTGGG 1921 TACCAGCAAC ATGGATTTCC TCAGATACTG AGTCTCAATT GCCTACTTTC TTGTACAAAG 1981 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2041 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2101 ACGACGCAAC GGTACAAGAG TTCCCATCAC GCGTTAAGTC gacaatcaac ctctggatta 2161 caaaatttgt gaaagatt