Construct: ORF TRCN0000473225
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004545.1_s317c1
- Derived from:
- ccsbBroadEn_11408
- DNA Barcode:
- GCGTCAGGGTCGACTATTGATCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIAA0408 (9729)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473225
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9729 | KIAA0408 | KIAA0408 | NM_014702.5 | 83% | 83.1% | 1_351del;702C>T;2067C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1797
- ORF length:
- 1731
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg taaggaacaa aaagcaacaa aaaaatcaaa agtagggttt ttggatcctt 121 tggctacaga caaccaaaag gaatgtgagg cctggcctga cctgaggact tctgaggaag 181 acagcaagag ctgttctggc gccctcagta cagctcttga agaacttgcg aaggtgagtg 241 aagaattatg cagctttcaa gaggaaattc gaaagcggtc taaccataga aggatgaagt 301 cagattcttt tctccaggaa atgccaaatg taactaatat acctcatggg gaccccatga 361 tcaacaatga ccagtgcatt cttccaatca gtttagaaaa agaaaaacag aaaaatagga 421 agaatctgag ctgtaccaat gtgctccaga gcaattctac gaaaaaatgt ggaattgata 481 caatcgattt aaaaagaaat gaaactccac cagttcctcc tccaagaagc acctctcgaa 541 attttcccag ctcggattct gaacaagcct atgaaagatg gaaggaaagg ttagaccaca 601 acagctgggt gccccatgag ggtcgaagta aaaggaatta caaccctcac ttccctttga 661 gacaacaaga gatgtctatg ttgtatccaa atgaagggaa aacttcgaaa gatggtatca 721 tcttttcctc tttggtacca gaagtcaaaa tagatagcaa gcctccaagt aatgaagatg 781 ttggacttag catgtggtca tgtgacattg ggataggtgc aaaaaggagc ccctctactt 841 cgtggtttca gaaaacctgc tctaccccca gtaatccaaa atatgaaatg gtgatcccag 901 atcaccctgc taaatctcat cctgatcttc atgtaagtaa tgactgtagc tcctcagtag 961 cagagagcag tagcccactt agaaatttca gttgtggctt tgaaaggact acaaggaatg 1021 agaagctggc agcaaagact gatgaattta acagaactgt atttagaaca gatagaaatt 1081 gtcaggcaat acagcaaaat cacagctgct caaaatcatc ggaggatctc aagccctgtg 1141 atacctcatc tactcacaca ggtagcatat cacaaagtaa cgatgtgtcc ggtatttgga 1201 aaaccaatgc ccacatgcct gtgcccatgg aaaatgtgcc tgataatccc accaagaaat 1261 ccacaacagg cctagtaaga caaatgcagg gacaccTAAG TCCTCGCAGT TATCGAAATA 1321 TGCTCCACGA GCATGACTGG AGACCGAGTA ATTTGTCTGG CCGTCCGAGG TCAGCTGATC 1381 CCAGGTCAAA TTATGGTGTT GTGGAAAAGC TGCTGAAAAC CTATGAGACA GCAACAGAGT 1441 CTGCATTGCA AAATTCTAAG TGCTTCCAGG ATAATTGGAC CAAATGTAAT TCTGATGTCA 1501 GTGGTGGTGC CACATTAAGT CAGCATTTAG AAATGCTCCA AATGGAACAA CAGTTTCAGC 1561 AAAAGACAGC TGTGTGGGGG GGACAGGAAG TGAAGCAAGG AATAGATCCG AAAAAGATAA 1621 CAGAGGAATC CATGTCAGTG AACGCCTCAC ATGGAAAAGG ATTTTCCCGA CCTGCTAGAC 1681 CAGCAAATCG TCGTCTCCCC TCCAGATGGG CATCCAGATC TCCATCTGCA CCCCCTGCCT 1741 TGCGGAGAAC TACCCACAAC TATACCATTT CTCTGCGATC TGAAGCATTG ATGGTTTACC 1801 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1861 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1921 ATATATCTTG TGGAAAGGAC GAGCGTCAGG GTCGACTATT GATCACACGC GTTAAGTCga 1981 caatcaacct ctggattaca aaatttgtga aagatt