Construct: ORF TRCN0000473247
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006053.1_s317c1
- Derived from:
- ccsbBroadEn_10533
- DNA Barcode:
- GTAGGCTTTGACCCCCACGAAAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CTBP1-DT (92070)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473247
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92070 | CTBP1-DT | CTBP1 divergent transcript | NR_033339.1 | 9.2% | 1_1316del;1412T>G;1590_2945del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 119
- ORF end:
- 392
- ORF length:
- 273
- Sequence:
-
1 GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT TTTTCTTCCA 61 TTTCAGGTGT CGTGAGGCTA GCATCGATTG ATCAACAAGT TTGTACAAAA AAGTTGGCAT 121 GACCACCGCC CTCCAGGGTC AGCGTTTGGC TGTTTGTGTG CCTGCCCACC CGCCTCCCTG 181 TGCCTGGGCC TGCCCTGTGC CTGCACACAG GAGGGGCTCA TGGCGTTTGC CCTACACGGA 241 TGGGCTGTCC TGGGAGCTAC TGGACAGTCA CCTTGGTGGG AATGCCAGAG GCATGGGCAT 301 TAGGTCCCCC CGGCCAGCCT CCGTTGCCAC ATGGGCTATT TTTGTCCATC GCGTGGGACA 361 ACCTAGTATT GGGGGAAAAC TCAGTCCACT CTACCCAACT TTCTTGTACA AAGTGGTTGA 421 TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG 481 TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTA 541 GGCTTTGACC CCCACGAAAG CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 601 tgtgaaagat t