Construct: ORF TRCN0000473249
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010369.1_s317c1
- Derived from:
- ccsbBroadEn_12066
- DNA Barcode:
- TTTCCTAAACGAACACTTCTGCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HES2 (54626)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473249
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54626 | HES2 | hes family bHLH transcripti... | NM_019089.5 | 37.7% | 29.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 320
- ORF end:
- 548
- ORF length:
- 228
- Sequence:
-
1 TCAGCCGTCG CTTCATGTGA CTCCACGGAG TACCGGGCGC CGTCCAGGCA CCTCGATTAG 61 TTCTCGAGCT TTTGGAGTAC GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA TGCGATGGAG 121 TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC 181 TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG 241 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG 301 TTTGTACAAA AAAGTTGGCA TGGGGCTGCC TCGCCGGGCA GGGGACGCGG CGGAGCTGCG 361 CAAGAGCCTG AAGCCGCTGC TGGAGAAGCG CCGGCGCGCG CGCATCAACC AGAGCCTGAG 421 CCAGCTTAAG GGGCTCATCC TGCCGCTGCT GGGCCGGGAG GATGCTTCTG GCTGGCACAC 481 CTGGCTTCCC CTCCATGCTC AGAACTGCTT CCTACTCTAC ATCCAGGCTC CTGAGCAGCC 541 CCCAGCTTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC 601 TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT 661 TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTTCCTA AACGAACACT TCTGCTGACG 721 CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt