Construct: ORF TRCN0000473255
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011671.1_s317c1
- Derived from:
- ccsbBroadEn_04821
- DNA Barcode:
- TAGAGTCTAGATGGGACAACACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LYPLAL1 (127018)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473255
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 127018 | LYPLAL1 | lysophospholipase like 1 | NM_138794.5 | 99.8% | 99.5% | 393A>G |
| 2 | human | 127018 | LYPLAL1 | lysophospholipase like 1 | NM_001350628.1 | 96.2% | 95.9% | 188_214del;420A>G |
| 3 | human | 127018 | LYPLAL1 | lysophospholipase like 1 | XM_017000271.2 | 96.2% | 95.9% | 188_214del;420A>G |
| 4 | human | 127018 | LYPLAL1 | lysophospholipase like 1 | NM_001350629.1 | 96% | 95.3% | (many diffs) |
| 5 | human | 127018 | LYPLAL1 | lysophospholipase like 1 | NM_001300770.1 | 93.1% | 92.8% | 188_189ins48;345A>G |
| 6 | human | 127018 | LYPLAL1 | lysophospholipase like 1 | NM_001300771.1 | 83.8% | 83.5% | 0_1ins114;279A>G |
| 7 | human | 127018 | LYPLAL1 | lysophospholipase like 1 | NM_001300772.1 | 83.8% | 83.5% | 0_1ins114;279A>G |
| 8 | human | 127018 | LYPLAL1 | lysophospholipase like 1 | XM_011509183.2 | 80.7% | 80.4% | 0_1ins114;74_100del;306A>G |
| 9 | human | 127018 | LYPLAL1 | lysophospholipase like 1 | NM_001300769.1 | 78% | 75.5% | (many diffs) |
| 10 | human | 127018 | LYPLAL1 | lysophospholipase like 1 | XM_017000272.1 | 77% | 76.7% | 0_1ins114;74_75ins48;231A>G |
| 11 | human | 127018 | LYPLAL1 | lysophospholipase like 1 | XR_001736968.2 | 61% | (many diffs) | |
| 12 | human | 127018 | LYPLAL1 | lysophospholipase like 1 | XR_001736966.2 | 40.6% | (many diffs) | |
| 13 | human | 127018 | LYPLAL1 | lysophospholipase like 1 | XR_001736964.2 | 38% | (many diffs) | |
| 14 | human | 127018 | LYPLAL1 | lysophospholipase like 1 | XR_001736967.2 | 32.6% | 1_41del;434A>G;753_2174del | |
| 15 | human | 127018 | LYPLAL1 | lysophospholipase like 1 | XR_001736965.2 | 31.9% | (many diffs) | |
| 16 | human | 127018 | LYPLAL1 | lysophospholipase like 1 | XR_002959345.1 | 23.1% | 1_41del;434A>G;753_3073del | |
| 17 | human | 127018 | LYPLAL1 | lysophospholipase like 1 | XR_001736963.2 | 13.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 777
- ORF length:
- 711
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctgcgtcg gggtcggttc tgcagcgctg tatcgtgtcg ccggcaggga 121 ggcatagcgc ctctctgatc ttcctgcatg gctcaggtga ttctggacaa ggattaagaa 181 tgtggatcaa gcaggtttta aatcaagatt taacattcca acacataaaa attatttatc 241 caacagctcc tcccagatca tatactccta tgaaaggagg aaTCTCCAAT GTATGGTTTG 301 ACAGATTTAA AATAACCAAT GACTGCCCAG AACACCTTGA ATCAATTGAT GTCATGTGTC 361 AAGTGCTTAC TGATTTGATT GATGAAGAAG TAAAAAGTGG CATCAAGAAG AACAGGATAT 421 TAATAGGAGG ATTCTCTATG GGAGGATGCA TGGCAATGCA TTTAGCATAT AGAAATCATC 481 AAGATGTGGC AGGAGTATTT GCTCTTTCTA GTTTTCTGAA TAAAGCATCT GCTGTTTACC 541 AGGCTCTTCA GAAGAGTAAT GGTGTACTTC CTGAATTATT TCAGTGTCAT GGTACTGCAG 601 ATGAGTTAGT TCTTCATTCT TGGGCAGAAG AGACAAACTC AATGTTAAAA TCTCTAGGAG 661 TGACCACGAA GTTTCATAGT TTTCCAAATG TTTACCATGA GCTAAGCAAA ACTGAGTTAG 721 ACATATTGAA GTTATGGATT CTTACAAAGC TGCCAGGAGA AATGGAAAAA CAAAAATGCC 781 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 841 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 901 ATATATCTTG TGGAAAGGAC GATAGAGTCT AGATGGGACA ACACCTACGC GTTAAGTCga 961 caatcaacct ctggattaca aaatttgtga aagatt