Construct: ORF TRCN0000473297

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001804.1_s317c1
Derived from:
ccsbBroadEn_07942
DNA Barcode:
CAAACGATTTGTCCTTAAATTCTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FSCN2 (25794)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473297
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 25794 FSCN2 fascin actin-bundling prote... NM_012418.4 99.8% 100% 705A>T;1446C>T
2 human 25794 FSCN2 fascin actin-bundling prote... NM_001077182.3 95.2% 95.3% 705A>T;1105_1176del;1518C>T
3 human 25794 FSCN2 fascin actin-bundling prote... XM_011524587.2 95.2% 95.3% 705A>T;1105_1176del;1518C>T
4 human 25794 FSCN2 fascin actin-bundling prote... XM_011524590.2 95.2% 95.3% 705A>T;1105_1176del;1518C>T
5 mouse 238021 Fscn2 fascin actin-bundling prote... NM_172802.4 85.7% 90.8% (many diffs)
6 mouse 238021 Fscn2 fascin actin-bundling prote... XM_006533259.3 81.5% 86.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1545
ORF length:
1476
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gccgacgaac ggcctgcacc aggtgctgaa gatccagttt ggcctcgtca
121acgacactga ccgctacctg acagctgaga gcttcggctt caaggtcaat gcctcggcac
181ccagcctcaa gaggaagcag acctgggtgc tggaacccga cccaggacaa ggcacggctg
241tgctgctccg cagcagccac ctgggccgct acctgtcggc agaagaggac gggcgcgtgg
301cctgtgaggc agagcagccg ggccgtgact gccgcttcct ggtcctgccg cagccagatg
361ggcgctgggt gctgcggtcc gagccgcacg gccgcttctt cggaggcacc gaggaccagc
421tgtcctgctt cgccacagcc gtttccccgg ccgagctgtg gaccgtgcac ctggccatcc
481acccgcaggc ccacctgctg agcgtgagcc ggcggcgcta cgtgcacctg tgcccgcggg
541aggacgagat ggccgcagac ggagacaagc cctggggcgt ggacgccctc ctcaccctca
601tcttccggag ccgacggtac tgcctcaagt cctgtgacag ccgctacctg cgcagcgacg
661gccgtctggt ctgggagcct gagccccgtg cctgctacac gctggagttc aaggcgggca
721agctggcctt caaggactgc gacggccact acctggcacc cgtggggccc gctggcaccc
781tcaaggccgg ccgaaacacg cgacctggca aggatgagct ctttgatctg gaggagagtc
841acccacaggt ggtgctggtg gctgccaacc accgctacgt ctctgtgcgg caaggggtca
901acgtctcagc caatcaggat gatgaactag accacgagac cttcctgatg caaattgacc
961aggagacaaa gaagtgcacc ttctattcca gcactggggg ctactggacc ctggtcaccc
1021atgggggcat tcacgccaca gccacacaag tttctgccaa caccatgttt gagatggagt
1081ggcgtggccg gcgggtagca ctcaaagcca gcaacgggcg cTACGTGTGC ATGAAGAAGA
1141ATGGGCAGCT GGCGGCTATC AGCGATTTTG TCGGCAAGGA CGAAGAGTTC ACCCTCAAGC
1201TCATCAACCG GCCCATCCTG GTGCTGCGCG GCCTGGACGG CTTCGTCTGC CACCACCGCG
1261GCTCCAACCA GCTGGACACC AACCGCTCCG TCTACGACGT CTTCCACCTG AGCTTCAGCG
1321ACGGCGCCTA CCGGATCCGA GGCCGCGACG GAGGGTTCTG GTACACGGGC AGCCACGGCA
1381GCGTGTGCAG CGACGGCGAA CGCGCCGAGG ACTTCGTCTT CGAGTTCCGT GAGCGCGGCC
1441GCCTGGCCAT CCGCGCCCGG AGCGGCAAGT ACCTGCGCGG CGGCGCCTCG GGCCTGCTGC
1501GGGCCGATGC CGATGCCCCG GCCGGGACCG CGCTTTGGGA GTACTTGCCA ACTTTCTTGT
1561ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1621AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1681GAAAGGACGA CAAACGATTT GTCCTTAAAT TCTTACGCGT TAAGTCgaca atcaacctct
1741ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)

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