Construct: ORF TRCN0000473316
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008265.1_s317c1
- Derived from:
- ccsbBroadEn_04060
- DNA Barcode:
- GAAGTCTGGCTCCAAAACCCCGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOL12 (79159)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473316
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79159 | NOL12 | nucleolar protein 12 | NM_024313.3 | 100% | 100% | |
| 2 | mouse | 97961 | Nol12 | nucleolar protein 12 | NM_133800.3 | 85.1% | 89.4% | (many diffs) |
| 3 | mouse | 97961 | Nol12 | nucleolar protein 12 | XM_006521607.2 | 56.8% | 58.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ccgcaacaag aagaagaagc gagatggtga cgaccggcgg ccgaggctcg 121 ttcttagctt cgacgaggag aagaggcggg agtacctgac aggcttccac aagcggaagg 181 tcgagcgaaa gaaggcagcc attgaggaga ttaagcagcg gctgaaagag gagcagagga 241 agcttcggga ggAGCGCCAC CAGGAATACT TGAAGATGCT GGCAGAGAGA GAAGAGGCTC 301 TGGAGGAGGC AGATGAGCTG GACCGGTTGG TGACAGCAAA GACGGAGTCG GTGCAGTATG 361 ACCACCCCAA CCACACAGTC ACCGTGACCA CCATCAGTGA CCTGGACCTC TCGGGGGCCC 421 GGCTGCTCGG GCTGACCCCA CCTGAGGGAG GGGCTGGAGA CAGGTCTGAG GAGGAGGCGT 481 CATCCACGGA GAAACCAACC AAAGCCTTGC CCAGGAAGTC CAGAGACCCC CTGCTCTCTC 541 AGCGGATCTC CTCCCTCACA GCATCACTAC ATGCACACAG CCGCAAAAAG GTCAAGAGGA 601 AACATCCCCG ACGGGCCCAG GACTCCAAAA AGCCCCCAAG GGCCCCTCGT ACCAGCAAGG 661 CCCAGCGCCG CCGTCTCACA GGCAAAGCAC GGCACAGCGG GGAGTGCCCA ACTTTCTTGT 721 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 781 AGTAATGAAC TAGCCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 841 GAAAGGACGA GAAGTCTGGC TCCAAAACCC CGTGACGCGT TAAGTCgaca atcaacctct 901 ggattacaaa atttgtgaaa gatt