Construct: ORF TRCN0000473329
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012497.1_s317c1
- Derived from:
- ccsbBroadEn_01824
- DNA Barcode:
- GTGAAGGGTTAGCTAGACCTTTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC30A1 (7779)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473329
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7779 | SLC30A1 | solute carrier family 30 me... | NM_021194.3 | 100% | 100% | |
| 2 | mouse | 22782 | Slc30a1 | solute carrier family 30 (z... | NM_009579.3 | 82.9% | 85.4% | (many diffs) |
| 3 | mouse | 22782 | Slc30a1 | solute carrier family 30 (z... | XM_006497158.3 | 35% | 35.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1590
- ORF length:
- 1521
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggtgttgg ggtcggaacc ggggccggct gctgtgcatg ctggcgctga 121 ccttcatgtt catggtgctg gaggtggtgg tgagccgggt gacctcgtcg ctggcgatgc 181 tctccgactc cttccacatg ctgtcggacg tgctggcgct ggtggtggcg ctggtggccg 241 agcgcttcgc ccggcggacc cacgccaccc agaagaacac gttcggctgg atccgagccg 301 aggtaatggg ggctctggtg aacgccatct tcctgactgg cctctgtttc gccatcctgc 361 tggaggccat cgagcgcttc atcgagccgc acgagatgca gcagccgctg gtggtccttg 421 gggtcggcgt ggccgggctg ctggtcaacg tgctggggct ctgcctcttc caccatcaca 481 gcggcttcag ccaggactcc ggccacggcc actcgcacgg gggtcacggc cacggccacg 541 gcctccccaa ggggcctcgc gttaagagca cccgccccgg gagcagcgac atcaacgtgg 601 ccccgggcga gcagggtccc gaccaggagg agaccaacac cctggtggcc aataccagca 661 actccaacgg gctgaaattg gaccccgcag acccagaaaa ccccagaagt ggtgatacag 721 tggaagtaca agtgaatgga aatcttgtca gagaacctga ccatatggaa ctggaagaag 781 atagggctgg acaacttaac atgcgtggag tttttctgca tgtccttgga gatgccttgg 841 gttcagtgat tgtagtagta aatgccttag tcttttactt ttcttggaaa ggttgttctg 901 aaggggattt ttgtgtgaat ccatgtttcc ctgacccctg caaagcattt gtagaaataa 961 ttaatagtac tcatgcatca gtttatgagg ctggtccttg ctgggtgcta tatttagatc 1021 caactctttg tgttgtaatg gtttgtatac ttctttacac aacctatcca ttacttaagg 1081 aatctgctct tattcttcta caaactgttc ctaaacaaat tgatatcaga aatttgataa 1141 aagaacttcg aaatgttGAA GGAGTTGAGG AAGTTCATGA ATTACATGTT TGGCAACTTG 1201 CTGGAAGCAG AATCATTGCC ACTGCTCACA TAAAATGTGA AGATCCAACA TCATACATGG 1261 AGGTGGCTAA AACCATTAAA GACGTTTTTC ATAATCACGG AATTCACGCT ACTACCATTC 1321 AGCCTGAATT TGCTAGTGTA GGCTCTAAAT CAAGTGTAGT TCCGTGTGAA CTTGCCTGCA 1381 GAACCCAGTG TGCTTTGAAG CAATGTTGTG GGACACTACC ACAAGCCCCT TCTGGAAAGG 1441 ATGCAGAAAA GACCCCAGCA GTTAGCATTT CTTGTTTAGA ACTTAGTAAC AATCTAGAGA 1501 AGAAGCCCAG GAGGACTAAA GCTGAAAACA TCCCTGCTGT TGTGATAGAG ATTAAAAACA 1561 TGCCAAACAA ACAACCTGAA TCATCTTTGT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1621 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGCC 1681 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGTGAA 1741 GGGTTAGCTA GACCTTTCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1801 tgaaagatt