Construct: ORF TRCN0000473364
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003260.1_s317c1
- Derived from:
- ccsbBroadEn_06909
- DNA Barcode:
- ATTATAACATTAATATTATTGGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SAA2 (6289)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473364
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6289 | SAA2 | serum amyloid A2 | NM_030754.5 | 99.4% | 99.1% | 216T>C;266G>A |
| 2 | human | 6288 | SAA1 | serum amyloid A1 | NM_000331.5 | 97.2% | 95% | (many diffs) |
| 3 | human | 6288 | SAA1 | serum amyloid A1 | NM_001178006.2 | 97.2% | 95% | (many diffs) |
| 4 | human | 6288 | SAA1 | serum amyloid A1 | NM_199161.5 | 96.9% | 94.2% | (many diffs) |
| 5 | human | 6289 | SAA2 | serum amyloid A2 | NM_001127380.3 | 65.2% | 63.1% | (many diffs) |
| 6 | human | 100528017 | SAA2-SAA4 | SAA2-SAA4 readthrough | NM_001199744.2 | 54.3% | 48.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 432
- ORF length:
- 366
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcttctcacg ggcctggttt tctgctcctt ggtcctgagt gtcagcagcc 121 gaagcttctt ttcgttcctt ggcgaggctt tTGATGGGGC TCGGGACATG TGGAGAGCCT 181 ACTCTGACAT GAGAGAAGCC AATTACATCG GCTCAGACAA ATACTTCCAT GCTCGGGGGA 241 ACTATGATGC TGCCAAAAGG GGACCTGGGG GTGCCTGGGC CGCAGAAGTG ATCAGCAATG 301 CCAGAGAGAA TATCCAGAGA CTCACAGGCC ATGGTGCGGA GGACTCGCTG GCCGATCAGG 361 CTGCCAATAA ATGGGGCAGG AGTGGCAGAG ACCCCAATCA CTTCCGACCT GCTGGCCTGC 421 CTGAGAAATA CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 481 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 541 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATT ATAACATTAA TATTATTGGT 601 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t