Construct: ORF TRCN0000473385
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013029.1_s317c1
- Derived from:
- ccsbBroadEn_07025
- DNA Barcode:
- AGTAAGTCAACGCACACAACATGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYP (6855)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473385
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6855 | SYP | synaptophysin | NM_003179.2 | 99.8% | 99.6% | 587C>T |
| 2 | mouse | 20977 | Syp | synaptophysin | NM_009305.2 | 90.1% | 94.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1005
- ORF length:
- 939
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gctgctggcg gacatggacg tggtgaatca gctggtggct gggggtcagt 121 tccgggtggt caaggagccc ctcggctttg tgaaggtgct gcaatgggtc ttcgccatct 181 tcgcctttgc cacatgcggc agctacagtg gggagctcca gctgagcgtg gattgtgcca 241 acaagaccga gagtgacctc agcatcgagg tcgagttcga gtaccccttc aggctgcacc 301 aagtgtactt tgatgcaccc acctgccgag ggggcaccac caaggtcttc ttagttgggg 361 actactcctc gtcagccgaa ttctttgtca ccgtggccgt gtttgccttc ctctactcca 421 tgggggctct ggccacctac atcttcctgc agaacaagta ccgagagaat aacaaagggc 481 ccatgctgga ctttctggcc acggctgtgt tcgccttcat gtggctagtt AGCTCATCGG 541 CATGGGCCAA GGGGCTGTCA GATGTGAAGA TGGCCACAGA CCCAGAGAAC ATTATCAAGG 601 AGATGCCTGT CTGCCGCCAG ACAGGGAACA CATGCAAGGA GCTGAGAGAC CTTGTGACCT 661 CGGGACTCAA CACCTCGGTG GTGTTCGGCT TCCTGAACCT GGTGCTCTGG GTCGGCAACC 721 TGTGGTTCGT GTTTAAGGAG ACAGGCTGGG CCGCCCCGTT CCTGCGCGCG CCTCCCGGCG 781 CCCCCGAGAA ACAACCGGCA CCCGGGGACG CCTACGGCGA TGCAGGCTAC GGGCAGGGCC 841 CCGGCGGGTA CGGGCCCCAG GATTCCTACG GGCCTCAGGG CGGCTACCAG CCTGACTATG 901 GTCAACCAGC CGGCAGCGGT GGCAGTGGCT ACGGGCCTCA GGGCGACTAT GGGCAGCAAG 961 GCTACGGCCC GCAGGGTGCA CCCACCTCCT TCTCCAATCA GATGTACCCA ACTTTCTTGT 1021 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1081 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1141 GAAAGGACGA AGTAAGTCAA CGCACACAAC ATGAACGCGT TAAGTCgaca atcaacctct 1201 ggattacaaa atttgtgaaa gatt