Construct: ORF TRCN0000473401
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017210.1_s317c1
- Derived from:
- ccsbBroadEn_06353
- DNA Barcode:
- CTCTCCGATAATGGGCGGGCAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HBB (3043)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473401
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3043 | HBB | hemoglobin subunit beta | NM_000518.5 | 99.5% | 100% | 9T>C;153T>C |
| 2 | human | 3045 | HBD | hemoglobin subunit delta | NM_000519.4 | 92.7% | 93.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 531
- ORF length:
- 441
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGTGCACCT GACTCCTGAG GAGAAGTCTG 121 CCGTTACTGC CCTGTGGGGC AAGGTGAACG TGGATGAAGT TGGTGGTGAG GCCCTGGGCA 181 GGCTGCTGGT GGTCTACCCT TGGACCCAGA GGTTCTTTGA GTCCTTTGGG GATCTGTCCA 241 CCCCTGATGC TGTTATGGGC AACCCTAAGG TGAAGGCTCA TGGCAAGAAA GTGCTCGGTG 301 CCTTTAGTGA TGGCCTGGCT CACCTGGACA ACCTCAAGGG CACCTTTGCC ACACTGAGTG 361 AGCTGCACTG TGACAAGCTG CACGTGGATC CTGAGAACTT CAGGCTCCTG GGCAACGTGC 421 TGGTCTGTGT GCTGGCCCAT CACTTTGGCA AAGAATTCAC CCCACCAGTG CAGGCTGCCT 481 ATCAGAAAGT GGTGGCTGGT GTGGCTAATG CCCTGGCCCA CAAGTATCAC TACCCAACTT 541 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 601 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 661 CTTGTGGAAA GGACGACTCT CCGATAATGG GCGGGCAATT ACGCGTTAAG TCgacaatca 721 acctctggat tacaaaattt gtgaaagatt