Construct: ORF TRCN0000473417
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004105.1_s317c1
- Derived from:
- ccsbBroadEn_07062
- DNA Barcode:
- ATACACAGTGGTTTGCCCCCTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TGM3 (7053)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473417
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7053 | TGM3 | transglutaminase 3 | NM_003245.4 | 99.9% | 100% | 2073A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2148
- ORF length:
- 2079
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgctcta ggagtccaga gtatcaactg gcagacggcc ttcaaccgac 121 aagcgcatca cacagacaag ttctccagcc aggagctcat cttgcggaga ggccaaaact 181 tccaggtctt aatgatcatg aacaaaggcc ttggctctaa cgaaagactg gagttcattg 241 tctccacagg gccttacccc tcagagtcgg ccatgacgaa ggctgtgttt ccactctcca 301 atggcagtag tggtggctgg agtgcggtgc ttcaggccag caatggcaat actctgacta 361 tcagcatctc cagtcctgcc agcgcaccca taggacggta cacaatggcc ctccagatct 421 tctcccaggg cggcatctcc tctgtgaaac ttgggacgtt catactgctt tttaacccct 481 ggctgaatgt ggatagcgtc tttatgggta accacgctga gagagaagag tatgttcagg 541 aagatgccgg catcatcttt gtgggaagca caaaccgaat tggcatgatt ggctggaact 601 ttggacagtt tgaagaagac attctcagca tctgcctctc aatcttggat aggagtctga 661 atttccgccg tgacgctgct actgatgtgg ccagcagaaa tgaccccaaa tacgttggcc 721 gggtgctgag tgccatgatc aatagcaatg atgacaatgg tgtgcttgct gggaattgga 781 gcggcactta caccggtggc cgggacccaa ggagctggaa cggcagcgtg gagatcctca 841 aaaattggaa aaaatctggc ttcagcccag tccgatatgg ccagtgctgg gtctttgctg 901 ggaccctcaa cacagcgctg cggtctttgg ggattccttc ccgggtgatc accaacttca 961 actcagctca tgacacagac cgaaatctca gtgtggatgt gtactacgac cccatgggaa 1021 accccctgga caagggtagt gatagcgtat ggaatttcca tgtctggaat gaaggctggt 1081 ttgtgaggtc tgacctgggc ccctcgtacg gtggatggca ggtgttggat gctaccccgc 1141 aggaaagaag ccaaggggtg ttccagtgcg gccccgcttc ggtcattggt gttcgagagg 1201 gtgatgtgca gctgaacttc gacatgccct ttatcttcgc ggaggttaat gccgaccgca 1261 tcacctggct gtacgacaac accactggca aacagtggaa gaattccgtg aacagtcaca 1321 ccattggcag gtacatcagc accaaggcgg tgggcagcaa tgctcgcatg gacgtcacgg 1381 acaagtacaa gtacccagaa ggctctgacc aggaaagaca agtgttccaa aaggctttgg 1441 ggaaacttaa acccaacacg ccatttgccg cgacgtcttc aatgggtttg gaaacagagg 1501 aacaggagcc cagcatcatc gggaagctga aggtcgctgg catgctggca gtaggcaaag 1561 aagtcaacct ggtcctactg ctcaaaaacc tgagcaggga tacgaagaca gtgacagtga 1621 acatgacagc ctggaccatc atctacaacg gcacgcttgt acatgaagtg tggaaggact 1681 ctgccacaat gtccctggac cctgaggaag aggcagaaca tcccataaag atctcgtacg 1741 ctcagtatga gaagtacctg aagtcagaca acatgatccg gatcacagcg gtgtgcaagg 1801 tcccagatga gtctgaggtg gtggtggagc gggacatcat cctggacaac cccaccttga 1861 ccctGGAGGT GCTGAACGAG GCTCGTGTGC GGAAGCCTGT GAACGTGCAG ATGCTCTTCT 1921 CCAATCCACT GGATGAGCCG GTGAGGGACT GCGTGCTGAT GGTGGAGGGA AGCGGCCTGC 1981 TGTTGGGTAA CCTGAAGATC GACGTGCCGA CCCTAGGGCC CAAGGAGGGG TCCCGGGTCC 2041 GTTTTGATAT CCTGCCCTCC CGGAGTGGCA CCAAGCAACT GCTCGCCGAC TTCTCCTGCA 2101 ACAAGTTCCC TGCAATCAAG GCCATGTTGT CCATCGATGT GGCCGAATTG CCAACTTTCT 2161 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2221 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2281 GTGGAAAGGA CGAATACACA GTGGTTTGCC CCCTTATACG CGTTAAGTCg acaatcaacc 2341 tctggattac aaaatttgtg aaagatt