Construct: ORF TRCN0000473486
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009269.1_s317c1
- Derived from:
- ccsbBroadEn_11453
- DNA Barcode:
- GAACTTAAAACCGTGTTTACTCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EDIL3 (10085)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473486
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10085 | EDIL3 | EGF like repeats and discoi... | NM_001278642.1 | 99.9% | 100% | 921G>A |
| 2 | human | 10085 | EDIL3 | EGF like repeats and discoi... | NM_005711.5 | 97.8% | 97.9% | 196_225del;951G>A |
| 3 | mouse | 13612 | Edil3 | EGF-like repeats and discoi... | NM_010103.4 | 89.8% | 95.7% | (many diffs) |
| 4 | mouse | 13612 | Edil3 | EGF-like repeats and discoi... | NM_001037987.3 | 87.9% | 93.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1476
- ORF length:
- 1410
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcgctcggta gccgtctggc tcttggtcgg gctcagcctc ggtgtccccc 121 agttcggcaa aggtgatatt tgtgatccca atccatgtga aaatggaggt atctgtttgc 181 caggattggc tgatggttcc ttttcctgtg agtgtccaga tggcttcaca gaccccaact 241 gttctagtgt tgtggaggtt ggtccctgca ctcctaatcc atgccataat ggaggaacct 301 gtgaaataag tgaagcatac cgaggggata cattcatagg ctatgtttgt aaatgtcccc 361 gaggatttaa tgggattcac tgtcagcaca acataaatga atgcgaagtt gagccttgca 421 aaaatggtgg aatatgtaca gatcttgttg ctaactattc ctgtgagtgc ccaggcgaat 481 ttatgggaag aaattgtcaa tacaaatgct caggcccact gggaattgaa ggtggaatta 541 tatcaaacca gcaaatcaca gcttcctcta ctcaccgagc tctttttgga ctccaaaaat 601 ggtatcccta ctatgcacgt cttaataaga aggggcttat aaatgcgtgg acagctgcag 661 aaaatgacag atggccgtgg attcagataa atttgcaaag gaaaatgaga gttactggtg 721 tgattaccca aggagccaag aggattggaa gcccagagta tataaaatcc tacaaaattg 781 cctacagtaa tgatggaaag acttgggcaa tgtacaaagt gaaaggcacc aatgaagaca 841 tggtgtttcg tggaaacatt gataacaaca ctccatatgc taactctttc acacccccca 901 taaaagctca gtatgtaaga ctctatcccc aagtttgtcg aagacattgc actttgcgaa 961 tggaacttct tggctgtgaa ctgtcaggtt gttctgagcc tctgggtatg aaatcaggac 1021 atatacaaga ctatcagatc actgccTCCA GCATCTTCAG AACGCTCAAC ATGGACATGT 1081 TCACTTGGGA ACCAAGGAAA GCTCGGCTGG ACAAGCAAGG CAAAGTGAAT GCCTGGACCT 1141 CTGGCCACAA TGACCAGTCA CAATGGTTAC AGGTGGATCT TCTTGTTCCA ACCAAAGTGA 1201 CTGGCATCAT TACACAAGGA GCTAAAGATT TTGGTCATGT ACAGTTTGTT GGCTCCTACA 1261 AACTGGCTTA CAGCAATGAT GGAGAACACT GGACTGTATA CCAGGATGAA AAGCAAAGAA 1321 AAGATAAGGT TTTCCAGGGA AATTTTGACA ATGACACTCA CAGAAAAAAT GTCATCGACC 1381 CTCCCATCTA TGCACGACAC ATAAGAATCC TTCCTTGGTC CTGGTACGGG AGGATCACAT 1441 TGCGGTCAGA GCTGCTGGGC TGCACAGAGG AGGAATGCCC AACTTTCTTG TACAAAGTGG 1501 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1561 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1621 AGAACTTAAA ACCGTGTTTA CTCAAACGCG TTAAGTCgac aatcaacctc tggattacaa 1681 aatttgtgaa agatt