Construct: ORF TRCN0000473507
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016050.1_s317c1
- Derived from:
- ccsbBroadEn_10328
- DNA Barcode:
- CAAACGGTCCGTGATCATAAGAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LINC00314 (246705)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473507
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 246705 | LINC00314 | long intergenic non-protein... | NR_027246.1 | 31.9% | 1_104del;291_583del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 282
- ORF end:
- 468
- ORF length:
- 186
- Sequence:
-
1 GTCCAGGCAC CTCGATTAGT TCTCGAGCTT TTGGAGTACG TCGTCTTTAG GTTGGGGGGA 61 GGGGTTTTAT GCGATGGAGT TTCCCCACAC TGAGTGGGTG GAGACTGAAG TTAGGCCAGC 121 TTGGCACTTG ATGTAATTCT CCTTGGAATT TGCCCTTTTT GAGTTTGGAT CTTGGTTCAT 181 TCTCAAGCCT CAGACAGTGG TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGCTA 241 GCATCGATTG ATCAACAAGT TTGTACAAAA AAGTTGGCAC CATGTTGAAG ATTGCAGTGA 301 TGGAAGTGTC TCAGTCAGCC TGGAAGACTG TATGGAAGGA GGTTGCCTGG CCACACTGTC 361 ATGTCCCCCT CTCCCTAAAA AAGGGACACA TTTGCCTTTG TGCAAGGCAA CACATACATT 421 TGGTCTGTGT ATATACTTTG GTCTATGTAT TCTGTTATTT AACATTCTTG CCAACTTTCT 481 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 541 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 601 GTGGAAAGGA CGACAAACGG TCCGTGATCA TAAGAGTACG CGTTAAGTCg acaatcaacc 661 tctggattac aaaatttgtg aaagatt