Construct: ORF TRCN0000473526
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006748.1_s317c1
- Derived from:
- ccsbBroadEn_04003
- DNA Barcode:
- GGTGCTGGCGCTCAACTCCCTCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FUNDC2 (65991)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473526
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65991 | FUNDC2 | FUN14 domain containing 2 | NM_023934.4 | 100% | 100% | |
| 2 | human | 388965 | FUNDC2P2 | FUN14 domain containing 2 p... | NR_003663.1 | 34.8% | (many diffs) | |
| 3 | mouse | 67391 | Fundc2 | FUN14 domain containing 2 | NM_026126.4 | 70.8% | 74% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 633
- ORF length:
- 567
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aacatctgcc ccacgtgccg gaagccaagt ggtggcgaca actgcgcgcc 121 actccgcggc ctaccgcgca gatcctctac gtgtgtcctc gcgagacaag ctcaccgaaa 181 tggccgcgtc cagtcaagga aactttgagg gaaattttga gtcactggac cttgcggaat 241 ttgctaagaa gcagccatgg tggcgtaagc tgttcgggca ggaatctgga ccttcagcag 301 aaaagtatag cgtggcaacc cagctgttca ttggaggtgt cactggatgg tgcacaggtt 361 tcatattcca gaaggttgga aagttggctg caacagctgt gggaggtgga ttttttctcc 421 ttcagcttgc aaaccatact gggtacatca aagttgactg gcaacgagtg gagaaggaca 481 tgaagaaagc caaagagcag ctgaagatcc gtaagagcaa tcagatacct actgaggtca 541 ggagcaaagc tgaggaggtg gtgtcatttg tgaagaagaa tgttctagta actgggggat 601 ttttcggagg ctttctgctt ggcatGGCAT CCTACCCAAC TTTCTTGTAC AAAGTGGTTG 661 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 721 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGG 781 TGCTGGCGCT CAACTCCCTC AAACGCGTTA AGTCgacaat caacctctgg attacaaaat 841 ttgtgaaaga tt