Construct: ORF TRCN0000473545

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018856.2_s317c1
Derived from:
ccsbBroadEn_14803
DNA Barcode:
CGAAATGCGCTGAAAGCACCGTTT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
MAPK8 (5599)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473545
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

No results found.

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
234
ORF length:
165
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggctgaccta atagatgggt actgccggct ggtgaatgga acctcgcagt
121catttatcat cagacctcag aaaggtgagg tgctgtttct gagcattccc aattgcgctt
181ctaccaaata tttagctgtg cttgccccta acagcctgtt atcctctttt aggtaactgt
241aaaaatgaag accaaatgaa taattcagct cagtataatc tgaggactta tttttctagg
301gatggtgatc cgtttcacac cttccacaag ccaagagctg ggtttgaatt gccaactttc
361ttgtacaaag ttggcattat aaganagctn ngtttgaatt gccaacttnc ttntacaann
421tnnngcaacg aacaggtcac tatcnntnan aanaaaanca ttgcttatca atattnnnnn
481nncngnnnan ntcnctatca gtatannnan nngtattaca nnntcatagc tgtttcctgg
541cagctctggc ccgtgtctca aaatntntgc tatccagntg ntncaaatcg aggcgccgaa
601caaaaaagct cctactattt tgttaaaaaa cccaagtcgt tctatagctg tggatgtgta
661tctaccacct cttcattctc gagacggcag agctgcccgc aaagcacgtt ttgctctagc
721actgatctct tcaaatcata caaaagctat tgacctctct tgttgtacta agttggtatt
781atacacagag tgattatcat ttgttgcttc gtagtgaccg tagagaaaaa tacactatta
841gtgtcccctg atgatcgccc gaccagagag tcatgaatct atggcagaac tcctcccaac
901agtaaggact tacgttgaaa acagacctaa atatgctgga tatagctttg agaaactctt
961ccctgatgtc cttttcccag ctgactcaga acacaacaaa cttaaagcca gtcaggcaag
1021ggatttgtta tccaaaatgc tggtaataga tgcatctaaa aggatctctg tagatgaagc
1081tctccaacac ccgtacatca atgtctggta tgatccTTCT GAAGCAGAAG CTCCACCACC
1141AAAGATCCCT GACAAGCAGT TAGATGAAAG GGAACACACA ATAGAAGAGT GGAAAGAATT
1201GATATATAAG GAAGTTATGG ACTTGGAGGA GAGAACCAAG AATGGAGTTA TACGGGGGCA
1261GCCCTCTCCT TTAGGTGCAG CAGTGATCAA TGGCTCTCAG CATCCATCAT CATCGTCGTC
1321TGTCAATGAT GTGTCTTCAA TGTCAACAGA TCCGACTTTG GCCTCTGATA CAGACAGCAG
1381TCTAGAAGCA GCAGCTGGGC CTCTGGGCTG CTGTAGATTG CCAACTTTCT TGTACAAAGT
1441GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG
1501AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA
1561CGACGAAATG CGCTGAAAGC ACCGTTTACG CGTTAAGTCg acaatcaacc tctggattac
1621aaaatttgtg aaagatt

Download FASTA (ORF) (Full)