Construct: ORF TRCN0000473563
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017808.1_s317c1
- Derived from:
- ccsbBroadEn_10914
- DNA Barcode:
- ATACCCACCTCAGGACAAAATTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGL (3535)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473563
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100423062 | IGLL5 | immunoglobulin lambda like ... | NM_001256296.2 | 53.2% | 48.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 768
- ORF length:
- 702
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctggaccgtt ctcctcctcg gcctcctctc tcactgcaca ggctctgtga 121 cctcctatgt gctgactcag ccaccctcgg tgtcagtggc cccaggacag acggccagga 181 ttacctgtgg gggaaacaac attggaagta aaagtgtgca ctggtaccag cagaagccag 241 gccaggcccc tgtgctggtc gtctatgatg atagcgaccg gccctcaggg atccctgagc 301 gattctctgg ctccaactct gggaacacgg ccaccctgac catcagcagg gtcgacgccg 361 gggatgaggC CGACTATTAC TGTCAGCTGT GGGATAGTAG TAGTGATCAT CCCGTAGTAT 421 TCGGCGGAGG GACCAAGCTG ACCGTCCTAG GTCAGCCCAA GGCTGCCCCC TCGGTCACTC 481 TGTTCCCGCC CTCCTCTGAG GAGCTTCAAG CCAACAAGGC CACACTGGTG TGTCTCATAA 541 GTGACTTCTA CCCGGGAGCC GTGACAGTGG CCTGGAAGGC AGATAGCAGC CCCGTCAAGG 601 CGGGAGTGGA GACCACCACA CCCTCCAAAC AAAGCAACAA CAAGTACGCG GCCAGCAGCT 661 ACCTGAGCCT GACGCCTGAG CAGTGGAAGT CCCACAGAAG CTACAGCTGC CAGGTCACGC 721 ATGAAGGGAG CACCGTGGAG AAGACAGTGG CCCCAACAGA ATGTTCATAC CCAACTTTCT 781 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 841 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 901 GTGGAAAGGA CGAATACCCA CCTCAGGACA AAATTTAACG CGTTAAGTCg acaatcaacc 961 tctggattac aaaatttgtg aaagatt