Construct: ORF TRCN0000473700
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007023.1_s317c1
- Derived from:
- ccsbBroadEn_05029
- DNA Barcode:
- GTTGAATTTACGTATCGTCCGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PYHIN1 (149628)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473700
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 149628 | PYHIN1 | pyrin and HIN domain family... | NM_198928.5 | 100% | 100% | |
| 2 | human | 149628 | PYHIN1 | pyrin and HIN domain family... | NM_152501.5 | 98.1% | 98.1% | 264_290del |
| 3 | human | 149628 | PYHIN1 | pyrin and HIN domain family... | XM_011509242.2 | 98.1% | 98.1% | 264_290del |
| 4 | human | 149628 | PYHIN1 | pyrin and HIN domain family... | NM_198930.4 | 93% | 91.9% | (many diffs) |
| 5 | human | 149628 | PYHIN1 | pyrin and HIN domain family... | XM_017000463.1 | 91.8% | 90.2% | (many diffs) |
| 6 | human | 149628 | PYHIN1 | pyrin and HIN domain family... | NM_198929.5 | 91.3% | 90.2% | (many diffs) |
| 7 | human | 149628 | PYHIN1 | pyrin and HIN domain family... | XM_011509243.2 | 90.7% | 90.2% | (many diffs) |
| 8 | human | 149628 | PYHIN1 | pyrin and HIN domain family... | XM_005244930.2 | 41.9% | 37.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1518
- ORF length:
- 1449
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcaaataac tacaagaaaa ttgttctact gaaaggatta gaggtcatca 121 atgattatca ttttagaatt gttaagtcct tactgagtaa cgatttaaaa cttaatccaa 181 aaatgaaaga agagtatgac aaaattcaga ttgctgactt gatggaggaa aagttcccag 241 gtgatgccgg tttgggcaaa ctaatagaat tcttcaaaga aataccaaca ctgggagacc 301 ttgctgaaac tcttaaaaga gaaaagttaa aagtcaaagg aataatccca tctaaaaaga 361 cgaaacagaa agaagtgtat cctgctacac ctgcatgcac cccaagcaac cgtctcacag 421 ctaaaggagc agaggagact cttggacctc agaaaagaaa aaaaccatct gaagaagaga 481 ctggaaccaa aaggagtaag atgtccaaag agcagactcg gccttcctgc tctgcaggag 541 ccagcacgtc cacagccatg ggccgttccc cacctcccca gacctcatca tcagctccac 601 ccaacacttc ctcaactgag agcctaaaac cattggccaa ccgtcacgca actgccagta 661 aaaatatttt ccgagaagac ccaataatcg cgatggtact aaatgcaaca aaagtattta 721 aatatgaatc ctcagaaaat gagcaaagaa gaatgtttca tgctacagtg gctacgcaga 781 cacagttctt tcatgtgaag gttttaaaca tcaacttgaa gaggaaattc attaaaaaga 841 gaatcatcat tatatcaaat tattccaaac gtaatagtct cctagaggtg aatgaagcct 901 cttctgtatc tgaagctggt cctgaccaaa cgtttgaggt tccaaaggac atcatcagaa 961 gagcaaagaa aattccGAAG ATCAATATTC TTCACAAACA AACTTCAGGA TATATTGTAT 1021 ATGGATTATT TATGCTACAT ACGAAAATTG TAAATAGGAA GACGACAATC TATGAAATTC 1081 AGGATAAAAC AGGAAGTATG GCTGTAGTAG GAAAAGGAGA ATGCCACAAT ATCCCCTGTG 1141 AAAAAGGAGA TAAGCTTCGA CTCTTCTGCT TTCGACTGAG AAAGAGGGAA AATATGTCAA 1201 AACTGATGTC AGAAATGCAT AGTTTCATCC AGATACAGAA AAATACAAAC CAGAGAAGCC 1261 ATGACTCCAG GAGCATGGCA CTACCCCAGG AACAGAGTCA GCATCCAAAA CCTTCAGAGG 1321 CCAGCACAAC CCTACCTGAA AGCCATCTCA AGACTCCTCA GATGCCACCA ACAACCCCAT 1381 CCAGCAGTTC CTTCACCAAG AAGGATGAAA CCCACCCAGG AGCACAGTCA TCGCCTGCAA 1441 ACTTTAGAAT CACCTCACCA ACTGTGGCCC CTCCTCTTTC TTCTGACACT TCCACCAACC 1501 GCCATCCAGC AGTTCCTTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1561 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1621 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGTTGAAT TTACGTATCG 1681 TCCGTAAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt