Construct: ORF TRCN0000473766
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002425.1_s317c1
- Derived from:
- ccsbBroadEn_00418
- DNA Barcode:
- AAGGTCCCAGTTCCATGGCACGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD55 (1604)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473766
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1604 | CD55 | CD55 molecule (Cromer blood... | NM_000574.5 | 100% | 100% | |
| 2 | human | 1604 | CD55 | CD55 molecule (Cromer blood... | NM_001300904.2 | 94.8% | 94.5% | (many diffs) |
| 3 | human | 1604 | CD55 | CD55 molecule (Cromer blood... | XM_017000467.2 | 89.9% | 85.8% | (many diffs) |
| 4 | human | 1604 | CD55 | CD55 molecule (Cromer blood... | NM_001300903.2 | 86.7% | 83.1% | 1081_1195del;1259_1317del |
| 5 | human | 1604 | CD55 | CD55 molecule (Cromer blood... | NM_001114752.3 | 86.5% | 80.4% | 1081_1198del;1262_1320del |
| 6 | human | 1604 | CD55 | CD55 molecule (Cromer blood... | NM_001300902.2 | 83.5% | 79.7% | (many diffs) |
| 7 | human | 1604 | CD55 | CD55 molecule (Cromer blood... | NR_125349.2 | 42.7% | 1_88del;1168_1252del;1317_2675del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1209
- ORF length:
- 1143
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cgtcgcgcgg ccgagcgtgc ccgcggcgct gcccctcctc ggggagctgc 121 cccggctgct gctgctggtg ctgttgtgcc tgccggccgt gtggggtgac tgtggccttc 181 ccccagatgt acctaatgcc cagccagctt tggaaggccg tacaagtttt cccgaggata 241 ctgtaataac gtacaaatgt gaagaaagct ttgtgaaaat tcctggcgag aaggactcag 301 tgatctgcct taagggcagt caatggtcag atattgaaga gttctgcaat cgtagctgcg 361 aggtgccaac aaggctaaat tctgcatccc tcaaacagcc ttatatcact cagaattatt 421 ttccagtcgg tactgttgtg gaatatgagt gccgtccagg ttacagaaga gaaccttctc 481 tatcaccaaa actaacttgc cttcagaatt taaaatggtc cacagcagtc gaattttgta 541 aaaagaaatc atgccctaat ccgggagaaa tacgaaatgg tcagattgat gtaccaggtg 601 gcatattatt tggtgcaacc atctccttct catgtaacac agggtacaaa ttatttggct 661 cgacttctag tttttgtctt atttcaggca gctctgtcca gtggagtgac ccgttgccag 721 agtgcagaga aatttattgt ccagcaccac cacaaattga caatggaata attcaagggg 781 aacgtgacca ttatggatat agacagtctg taacgtatgc atgtaataaa ggattcacca 841 tgattggaga gcactctatt tattgtactg tgaataatga tgaaggagag tggagtggcc 901 caccacctga atgcagagga aaatctctaa cttccaaggt cccaccaaca gttcagaaac 961 cTACCACAGT AAATGTTCCA ACTACAGAAG TCTCACCAAC TTCTCAGAAA ACCACCACAA 1021 AAACCACCAC ACCAAATGCT CAAGCAACAC GGAGTACACC TGTTTCCAGG ACAACCAAGC 1081 ATTTTCATGA AACAACCCCA AATAAAGGAA GTGGAACCAC TTCAGGTACT ACCCGTCTTC 1141 TATCTGGGCA CACGTGTTTC ACGTTGACAG GTTTGCTTGG GACGCTAGTA ACCATGGGCT 1201 TGCTGACTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1261 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1321 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAAGGTC CCAGTTCCAT GGCACGTGAC 1381 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt