Construct: ORF TRCN0000473952
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014538.1_s317c1
- Derived from:
- ccsbBroadEn_00957
- DNA Barcode:
- CGGATTCAATTTGAATGCTCTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- M6PR (4074)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473952
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4074 | M6PR | mannose-6-phosphate recepto... | NM_002355.4 | 100% | 100% | |
| 2 | human | 4074 | M6PR | mannose-6-phosphate recepto... | XM_005253376.2 | 100% | 100% | |
| 3 | human | 4074 | M6PR | mannose-6-phosphate recepto... | NM_001207024.1 | 68.9% | 68.9% | 453_454ins258 |
| 4 | mouse | 17113 | M6pr | mannose-6-phosphate recepto... | NM_010749.7 | 88.1% | 91.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 897
- ORF length:
- 831
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ccctttctac agctgctgga ggactggact gctactacta ctcctggctg 121 tggcagtgag agaatcctgg cagacagaag aaaaaacttg cgacttggta ggagaaaagg 181 gtaaagagtc agagaaagag ttggctctag tgaagaggct gaaaccactg tttaataaaa 241 gctttgagag cactgtgggc cagggttcag acacatacat ctacatcttc agggtgtgcc 301 gggaagctgg caaccacact tctggggcag gcctggtgca aatcaacaaa agtaatggga 361 aggagacagt ggtagggaga ctcaacgaga ctcacatctt caacggaagt aattggatca 421 tgctgatcta taaagggggt gatgaatatg acaaccactg tggCAAGGAG CAGCGTCGTG 481 CAGTGGTGAT GATCTCCTGC AATCGACACA CCCTAGCGGA CAATTTTAAC CCTGTGTCTG 541 AGGAGCGTGG CAAAGTCCAA GATTGTTTCT ACCTCTTTGA GATGGATAGC AGCCTGGCCT 601 GTTCACCAGA GATCTCCCAC CTCAGTGTGG GTTCCATCTT ACTTGTCACG TTTGCATCAC 661 TGGTTGCTGT TTATGTTGTT GGGGGGTTCC TATACCAGCG ACTGGTAGTG GGAGCCAAAG 721 GAATGGAGCA GTTTCCCCAC TTAGCCTTCT GGCAGGATCT TGGCAACCTG GTAGCAGATG 781 GCTGTGACTT TGTCTGCCGT TCTAAACCTC GAAATGTGCC TGCAGCATAT CGTGGTGTGG 841 GGGATGACCA GCTGGGGGAG GAGTCAGAAG AAAGGGATGA CCATTTATTA CCAATGTACC 901 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 961 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1021 ATATATCTTG TGGAAAGGAC GACGGATTCA ATTTGAATGC TCTTATACGC GTTAAGTCga 1081 caatcaacct ctggattaca aaatttgtga aagatt