Construct: ORF TRCN0000473999
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009058.1_s317c1
- Derived from:
- ccsbBroadEn_11695
- DNA Barcode:
- ACAAATCTAGTGTAGAACCATTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MLC1 (23209)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473999
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23209 | MLC1 | modulator of VRAC current 1 | XR_001755181.2 | 8.4% | 1_1610del;1806_2299del | |
| 2 | human | 23209 | MLC1 | modulator of VRAC current 1 | XR_001755180.2 | 7.7% | 1_1842del;2038_2531del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 320
- ORF end:
- 515
- ORF length:
- 195
- Sequence:
-
1 TCAGCCGTCG CTTCATGTGA CTCCACGGAG TACCGGGCGC CGTCCAGGCA CCTCGATTAG 61 TTCTCGAGCT TTTGGAGTAC GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA TGCGATGGAG 121 TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC 181 TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG 241 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG 301 TTTGTACAAA AAAGTTGGCA TGCATCTGTG GCCTGGGGCC ATCAAGATCA AAGAACCAGG 361 AGGCCTGGGA GATGCAGCTG GATGGGGCGG CCTGCAGACC CTGCCAGGGG GTTTGAGGAC 421 CCTCCCAGGT TTCCCACTGC GGAACAGGAG TGACTCTGGC TGCCAAGATA CCTTCATGGT 481 GTTCATGACA AGTGGAATCA TTATTTTCAA CCATTGCCCA ACTTTCTTGT ACAAAGTGGT 541 TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC 601 TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA 661 ACAAATCTAG TGTAGAACCA TTATACGCGT TAAGTCgaca atcaacctct ggattacaaa 721 atttgtgaaa gatt