Construct: ORF TRCN0000474032
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003792.1_s317c1
- Derived from:
- ccsbBroadEn_10397
- DNA Barcode:
- CAATACCAGAGGAAGACAATCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIR31HG (554202)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474032
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 554202 | MIR31HG | MIR31 host gene | NR_152878.1 | 11.5% | 1_181del;395_1852del | |
| 2 | human | 554202 | MIR31HG | MIR31 host gene | NR_152877.1 | 10.7% | 1_318del;532_1989del | |
| 3 | human | 554202 | MIR31HG | MIR31 host gene | NR_152879.1 | 10% | 1_440del;654_2111del | |
| 4 | human | 554202 | MIR31HG | MIR31 host gene | NR_027054.2 | 9.3% | 1_577del;791_2266del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 217
- ORF end:
- 430
- ORF length:
- 213
- Sequence:
-
1 GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA GACTGAAGTT AGGCCAGCTT 61 GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA GTTTGGATCT TGGTTCATTC 121 TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGCTAGC 181 ATCGATTGAT CAACAAGTTT GTACAAAAAA GTTGGCATGG ATTCCTGGAA ATACCTCCTC 241 AAGGCCAGTG TAGAGCCCTC AATCACCCAC TGCCCCCAGC TTCTCCTTCT CCCATCTCCT 301 GCACCAAGCA GGTCTCCAGG TGTTCCAGCT GCTGATGACG TAAAGTGTGG AGTTGGTCCA 361 GGAAGGTCAT GTTCCATGCA GCAGAGGAGC GCTTTGTGTG AGAAGTTGAA GACCTGCTGT 421 AACACATCAT ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC 481 CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG 541 ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACAATA CCAGAGGAAG ACAATCACCA 601 CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt