Construct: ORF TRCN0000474058
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006860.1_s317c1
- Derived from:
- ccsbBroadEn_12099
- DNA Barcode:
- GTACGGCCCTTAACAGGCCACCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPATCH4 (54865)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474058
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54865 | GPATCH4 | G-patch domain containing 4 | NM_182679.2 | 82.9% | 80.1% | 1067_1068insTG;1110_1111ins226 |
| 2 | human | 54865 | GPATCH4 | G-patch domain containing 4 | NM_015590.3 | 81.3% | 78% | (many diffs) |
| 3 | human | 54865 | GPATCH4 | G-patch domain containing 4 | XM_005245287.4 | 76.4% | 73.6% | 244_245ins87;980_981insTG;1023_1024ins226 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1404
- ORF length:
- 1338
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tgtcacccca gaggtcaaga gtcgtgggat gaagtttgct gaggagcagc 121 tgctaaagca tggatggact caaggcaaag gcctcggccg gaaggagaat ggtatcactc 181 aggctctcag ggtgacactg aagcaagaca ctcatggggt aggacatgac cctgccaagg 241 agttcacaaa ccactggtgg aatgagctct tcaacaagac tgcggccaac ttggtagtgg 301 aaactgggca ggatggagta cagataagga gcctttctaa ggagaccacc cgttataatc 361 atcccaagcc caacttgctg tatcagaagt ttgtgaagat ggctacattg acttcaggtg 421 gagagaagcc aaacaaagac ttggagagct gcagtgatga cgacaaccag gggtccaagt 481 ccccaaagat tctgactgat gagatgctgc tccaagcctg tgaggggcga acagcacaca 541 aggctgcccg tcttgggatc acaatgaagg ccaagcttgc tcgcctagag gcccaggagc 601 aggccttcct ggctcgtctc aaaggccagg accctggggc ccctcaactg cagtcagaga 661 gcaagccccc caaaaaaaag aaaaagaaaa ggaggcagaa agaggaggaa gaagctacag 721 catctgaaag gaatgatgca gatgagaagc acccagaaca tgctgagcag aacatcagaa 781 aaagcaagaa gaagaaaagg cgacatcaag aaggaaaggt ctcagatgaa agagagggta 841 caactaaagg gaatgagaag gaggacgctg caggaacaag tgggcttggg gaattgaata 901 gcagagagca aaccaatcag TCCCTCAGGA AAGGGAAGAA AAAGAAGAGG TGGCACCATG 961 AAGAGGAGAA GATGGGGGTC TTGGAGGAAG GAGGAAAAGG CAAGGAGGCT GCAGGCAGTG 1021 TCAGGACAGA GGAGGTAGAG AGCAGGGCAT ATGCTGACCC ATGCAGCCGA AGAAAGAAGA 1081 GGCAGCAACA GGAGGAGGAG GACTTGAACC TAGAAGATAG AGGTGAGGAA ACTGTTTTAG 1141 GTGGTGGAAC CAGGGAAGCA GAGAGCAGAG CATGCAGTGA TGGAAGAAGC AGGAAAAGCA 1201 AGAAGAAAAG ACAGCAGCAT CAAGAGGAGG AGGACATCTT GGATGTAAGG GATGAGAAGG 1261 ATGGCGGGGC TAGGGAAGCA GAGAGCAGAG CACACACTGG CTCAAGCAGC AGAGGTAAGA 1321 GGAAGAGGCA GCAGCATCCC AAGAAGGAAA GAGCTGGAGT CAGCACTGTC CAGAAAGCCA 1381 AAAAGAAACA GAAGAAGAGA GACTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1441 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1501 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG TACGGCCCTT 1561 AACAGGCCAC CAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1621 att