Construct: ORF TRCN0000474130
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000831.1_s317c1
- Derived from:
- ccsbBroadEn_13903
- DNA Barcode:
- TGGAACCGGTTAGCCGCTAGTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTAP (4507)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474130
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4507 | MTAP | methylthioadenosine phospho... | NM_002451.4 | 48.1% | 44% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 528
- ORF length:
- 462
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctctggcacc accaccaccg ccgtgaagat tggaataatt gGTGGAACAG 121 GCCTGGATGA TCCAGAAATT TTAGAAGGAA GAACTGAAAA ATATGTGGAT ACTCCATTTG 181 GCAAGCCATC TGATGCCTTA ATTTTGGGGA AGATAAAAAA TGTTGATTGC ATCCTCCTTG 241 CAAGGCATGG AAGGCAGCAC ACCATCATGC CTTCAAAGGT CAACTACCAG GCGAACATCT 301 GGGCTTTGAA GGAAGAGGGC TGTACACATG TCATAGTGAC CACAGCTTGT GGCTCCTTGA 361 GGGAGGAGAT TCAGCCCGGC GATATTGTCA TTATTGATCA GTTCATTGAC AGCCATGTAA 421 GACGTGCCTG CTTCCCCTTC ACCTTCCATC ATGATTGTTT CCAGAGACCT CCCCAGAAGC 481 CAAGCAGAAG CCACTGTGTT TCCTGTGCAA CCTGCACAAC CATGAGCTAC CCAACTTTCT 541 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 601 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 661 GTGGAAAGGA CGATGGAACC GGTTAGCCGC TAGTATCACG CGTTAAGTCg acaatcaacc 721 tctggattac aaaatttgtg aaagatt