Construct: ORF TRCN0000474143
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013832.1_s317c1
- Derived from:
- ccsbBroadEn_06829
- DNA Barcode:
- GTTATTATGCCCCAGTGGTTGAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB3B (5865)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474143
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5865 | RAB3B | RAB3B, member RAS oncogene ... | NM_002867.4 | 100% | 100% | |
| 2 | human | 5865 | RAB3B | RAB3B, member RAS oncogene ... | XM_017001958.1 | 100% | 100% | |
| 3 | mouse | 69908 | Rab3b | RAB3B, member RAS oncogene ... | NM_023537.5 | 89.3% | 97.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 723
- ORF length:
- 657
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttcagtgaca gatggtaaaa ctggagtcaa agatgcctct gaccagaatt 121 ttgactacat gtttaaactg cttatcattg gcaacagcag tgttggcaag acctccttcc 181 tcTTCCGCTA TGCTGATGAC ACGTTCACCC CAGCCTTCGT TAGCACCGTG GGCATCGACT 241 TCAAGGTGAA GACAGTCTAC CGTCACGAGA AGCGGGTGAA ACTGCAGATC TGGGACACAG 301 CTGGGCAGGA GCGGTACCGG ACCATCACAA CAGCCTATTA CCGTGGGGCC ATGGGCTTCA 361 TTCTGATGTA TGACATCACC AATGAAGAGT CCTTCAATGC TGTCCAAGAC TGGGCTACTC 421 AGATCAAGAC CTACTCCTGG GACAATGCAC AAGTTATTCT GGTGGGGAAC AAGTGTGACA 481 TGGAGGAAGA GAGGGTTGTT CCCACTGAGA AGGGCCAGCT CCTTGCAGAG CAGCTTGGGT 541 TTGATTTCTT TGAAGCCAGT GCAAAGGAGA ACATCAGTGT AAGGCAGGCC TTTGAGCGCC 601 TGGTGGATGC CATTTGTGAC AAGATGTCTG ATTCGCTGGA CACAGACCCG TCGATGCTGG 661 GCTCCTCCAA GAACACGCGT CTCTCGGACA CCCCACCGCT GCTGCAGCAG AACTGCTCAT 721 GCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 781 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 841 GGCTTTATAT ATCTTGTGGA AAGGACGAGT TATTATGCCC CAGTGGTTGA GCACGCGTTA 901 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt