Construct: ORF TRCN0000474147
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018175.1_s317c1
- Derived from:
- ccsbBroadEn_01098
- DNA Barcode:
- GCACCTCGTCGCAGATCACCGAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNU13 (4809)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474147
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4809 | SNU13 | small nuclear ribonucleopro... | NM_001003796.2 | 100% | 100% | |
| 2 | human | 4809 | SNU13 | small nuclear ribonucleopro... | NM_005008.4 | 100% | 100% | |
| 3 | mouse | 20826 | Snu13 | SNU13 homolog, small nuclea... | NM_011482.4 | 89.8% | 100% | (many diffs) |
| 4 | mouse | 100862468 | LOC100862468 | NHP2-like protein 1 | XM_003689185.5 | 89.8% | 100% | (many diffs) |
| 5 | mouse | 625931 | Gm6637 | predicted gene 6637 | NR_163697.1 | 59.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 450
- ORF length:
- 384
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggCATGAC TGAGGCTGAT GTGAATCCAA AGGCCTATCC CCTTGCCGAT GCCCACCTCA 121 CCAAGAAGCT ACTGGACCTC GTTCAGCAGT CATGTAACTA TAAGCAGCTT CGGAAAGGAG 181 CCAATGAGGC CACCAAAACC CTCAACAGGG GCATCTCTGA GTTCATCGTG ATGGCTGCAG 241 ACGCCGAGCC ACTGGAGATC ATTCTGCACC TGCCGCTGCT GTGTGAAGAC AAGAATGTGC 301 CCTACGTGTT TGTGCGCTCC AAGCAGGCCC TGGGGAGAGC CTGTGGGGTC TCCAGGCCTG 361 TCATCGCCTG TTCTGTCACC ATCAAAGAAG GCTCGCAGCT GAAACAGCAG ATCCAATCCA 421 TTCAGCAGTC CATTGAAAGG CTCTTAGTCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 481 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 541 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCACC 601 TCGTCGCAGA TCACCGAGGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 661 tgaaagatt