Construct: ORF TRCN0000474189
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010134.3_s317c1
- Derived from:
- ccsbBroadEn_14575
- DNA Barcode:
- CTAATTCACTATCTTAAATCTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDK5 (1020)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474189
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1020 | CDK5 | cyclin dependent kinase 5 | NM_004935.4 | 100% | 100% | |
| 2 | human | 1020 | CDK5 | cyclin dependent kinase 5 | NM_001164410.3 | 89% | 89% | 311_312ins96 |
| 3 | mouse | 12568 | Cdk5 | cyclin-dependent kinase 5 | NM_007668.3 | 90.8% | 99.6% | (many diffs) |
| 4 | mouse | 12568 | Cdk5 | cyclin-dependent kinase 5 | XM_006535626.2 | 83.4% | 90.4% | (many diffs) |
| 5 | mouse | 12568 | Cdk5 | cyclin-dependent kinase 5 | XR_389739.3 | 33% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 942
- ORF length:
- 876
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gaaatacgag aaactggaaa agattgggga aggcacctac ggaactgtgt 121 tcaaggccaa aaaccgggag actcatgaga tcgtggctct gaaacgggtg aggctggatg 181 acgatgatga gggtgtgccg agttccgccc tccgggagat ctgcctactc aaggagctga 241 agcacaagaa catcgtcagg cttcatgacg tcctgcacag cgacaagaag ctgactttgg 301 tttttgaatt ctgtgaccag gacctgaaga agtattttga cagttgcaat ggtgacctcg 361 atcctgagat tgtaaagtca ttcctcttcc agctactaaa agggctggga ttctgtcata 421 gccgcaatgt gctacacagg gacctgaagc cccagaacct gctaataaac aggaatgggg 481 agctgaaatt ggctgatttt ggccTGGCTC GAGCCTTTGG GATTCCCGTC CGCTGTTACT 541 CAGCTGAGGT GGTCACACTG TGGTACCGCC CACCGGATGT CCTCTTTGGG GCCAAGCTGT 601 ACTCCACGTC CATCGACATG TGGTCAGCCG GCTGCATCTT TGCAGAGCTG GCCAATGCTG 661 GGCGGCCTCT TTTTCCCGGC AATGATGTCG ATGACCAGTT GAAGAGGATC TTCCGACTGC 721 TGGGGACGCC CACCGAGGAG CAGTGGCCCT CTATGACCAA GCTGCCAGAC TATAAGCCCT 781 ATCCGATGTA CCCGGCCACA ACATCCCTGG TGAACGTCGT GCCCAAACTC AATGCCACAG 841 GGAGGGATCT GCTGCAGAAC CTTCTGAAGT GTAACCCTGT CCAGCGTATC TCAGCAGAAG 901 AGGCCCTGCA GCACCCCTAC TTCTCCGACT TCTGTCCGCC CTACCCAACT TTCTTGTACA 961 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1021 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1081 AGGACGACTA ATTCACTATC TTAAATCTCC CACGCGTTAA GTCgacaatc aacctctgga 1141 ttacaaaatt tgtgaaagat t