Construct: ORF TRCN0000474226
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011905.1_s317c1
- Derived from:
- ccsbBroadEn_05977
- DNA Barcode:
- GACGCCTTGCCGCCTGTAACGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDH17 (1015)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474226
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1015 | CDH17 | cadherin 17 | NM_001144663.2 | 99.7% | 99.7% | (many diffs) |
| 2 | human | 1015 | CDH17 | cadherin 17 | NM_004063.4 | 99.7% | 99.7% | (many diffs) |
| 3 | human | 1015 | CDH17 | cadherin 17 | XM_011516790.1 | 90.2% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2565
- ORF length:
- 2496
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatacttcag gcccatcttc actccctgtg tcttcttatg ctttatttgg 121 caactggata tggccaagag gggaagttta gtggacccct gaaacccatg acattttcta 181 tttatgaagg ccaagaaccg agtcaaatta tattccagtt taaggccaat cctcctgctg 241 tgacttttga actaactggg gagacagaca acatatttgt gatagaacgg gagggacttc 301 tgtattacaa cagagccttg gacagggaaa caagatctac tcacaatctc caggttgcag 361 ccctggacgc taatggaatt atagtggagg gtccagtccc tatcaccata gaagtgaagg 421 acatcaacga caatcgaccc acgtttctcc agtcaaagta cgaaggctca gtaaggcaga 481 actctcgccc aggaaagccc ttcttgtatg tcaatgccac agacctggat gatccggcca 541 ctcccaatgg ccagctttat taccagattg tcatccagct tcccatgatc aacaatgtta 601 tgtactttca gatcaacaac aaaacgggag ccatctctct tacccgagag ggatctcagg 661 aattgaatcc tgctaagaat ccttcctata atctggtgat ctcagtgaag gacatgggag 721 gccagagtga gaattccttc agtgatacca catctgtgga tatcatagtg acagagaata 781 tttggaaagc accaaaacct gtggagatgg tggaaaactc aactgatcct caccccatca 841 aaatcactca ggtgcggtgg aatgatcccg gtgcacaata ttccttagtt gacaaagaga 901 agctgccaag attcccattt tcaattgacc aggaaggaga tatttacgtg actcagccct 961 tggaccgaga agaaaaggat gcatatgttt tttatgcagt tgcaaaggat gagtacggaa 1021 aaccactttc atatccgctg gaaattcatg taaaagttaa agatattaat gataatccac 1081 ctacatgtcc gtcaccagta accgtatttg aggtccagga gaatgaacga ctgggtaaca 1141 gtatcgggac ccttactgca catgacaggg atgaagaaaa tactgccaac agttttctaa 1201 actacaggat tgtggagcaa actcccaaac ttcccatgga tggactcttc ctaatccaaa 1261 cctatgctgg aatgttacag ttagctaaac agtccttgaa gaagcaagat actcctcagt 1321 acaacttaac gatagaggtg tctgacaaag atttcaagac cctttgtttt gtgcaaatca 1381 acgttattga tatcaatgat cagatcccca tctttgaaaa atcagattat ggaaacctga 1441 ctcttgctga agacacaaac attgggtcca ccatcttaac catccaggcc actgatgctg 1501 atgagccatt tactgggagt tctaaaattc tgtatcatat cataaaggga gacagtgagg 1561 gacgcctggg ggttgacaca gatccccata ccaacaccgg atatgtcata attaaaaagc 1621 ctcttgattt tgaaacagca gctgtttcca acattgtgtt caaagcagaa aatcctgagc 1681 ctctagtgtt tggtgtgaag tacaatgcaa gttcttttgc caagttcacg cttattgtga 1741 cagatgtgaa tgaagcacct caattttccc aacacgtatt ccaagcgaaa gtcagtgagg 1801 atgtagctat aggcactaaa gtgggcaatg tgactgccaa ggatccagaa ggtctggaca 1861 taagttattc actgagggga gacacaagag gttggcttaa aattgaccac gtgactggtg 1921 agatctttag tgtggctcca ttggacagag aagccggaag tccatatcgg gtacaagtgg 1981 tggccacaga agtagggggg tcttccttga gctctgtgtc agagttccac ctgatcctta 2041 tggatgtgaa tgacaaccct CCCAGGCTAG CCAAGGACTA CACGGGCTTG TTCTTCTGCC 2101 ATCCCCTCAG TGCACCTGGA AGTCTCATTT TTGAGGCTAC TGATGATGAT CAGCACTTAT 2161 TTCGGGGTCC CCATTTTACA TTTTCCCTCG GCAGTGGAAG CTTACAAAAC GACTGGGAAG 2221 TTTCCAAAAT CAATGGTACT CATGCCCGAC TGTCTACCAG GCACACAGAC TTTGAGGAGA 2281 GGGAGTATGT CGTCTTGATC CGCATCAATG ATGGGGGTCG GCCACCCTTG GAAGGCATTG 2341 TTTCTTTACC AGTTACATTC TGCAGTTGTG TGGAAGGAAG TTGTTTCCGG CCAGCAGGTC 2401 ACCAGACTGG GATACCCACT GTGGGCATGG CAGTTGGTAT ACTGCTGACC ACCCTTCTGG 2461 TGATTGGTAT AATTTTAGCA GTTGTGTTTA TCCGCATAAA GAAGGATAAA GGCAAAGATA 2521 ATGTTGAAAG TGCTCAAGCA TCTGAAGTCA AACCTCTGAG AAGCTTGCCA ACTTTCTTGT 2581 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2641 AGTAATGAAC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GACGCCTTGC CGCCTGTAAC 2701 GCACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt