Construct: ORF TRCN0000474231
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001639.1_s317c1
- Derived from:
- ccsbBroadEn_13105
- DNA Barcode:
- CCCCTGCTGTAAGTCTGTGCTCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EARS2 (124454)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474231
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124454 | EARS2 | glutamyl-tRNA synthetase 2,... | NM_001308211.1 | 99.8% | 99.8% | 264G>A;606C>G;1369A>G |
| 2 | human | 124454 | EARS2 | glutamyl-tRNA synthetase 2,... | NM_001083614.2 | 94.9% | 94.1% | (many diffs) |
| 3 | human | 124454 | EARS2 | glutamyl-tRNA synthetase 2,... | XM_011545738.1 | 87.5% | 85.5% | (many diffs) |
| 4 | human | 124454 | EARS2 | glutamyl-tRNA synthetase 2,... | NR_003501.1 | 40.3% | (many diffs) | |
| 5 | human | 124454 | EARS2 | glutamyl-tRNA synthetase 2,... | XR_001751841.1 | 37.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1668
- ORF length:
- 1602
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgctcctg aggagactgc tgcagcgcga gaggccttcg gcggcctctg 121 gccgccccgt aggacggcgc gaggccaacc tgggcactga tgccggggtt gcggtgcgag 181 tgcggttcgc tcccagcccc acaggcttct tgcacctggg tggcctccgc actgccttgt 241 acaactacat ctttgctaag aagtaccagg ggagcttcat cctgaggcta gaggacacag 301 atcagactcg cgttgtgcct ggggcagcag agaatattga ggacatgctg gagtgggcag 361 gcatcccgcc tgatgagagc ccccgccggg gcggtcctgc tgggccctac cagcaatctc 421 agcggttgga gctgtatgcc caggccacag aagcgctgct gaagaccgga gctgcttacc 481 cctgtttctg ctcaccccag cggctggagc tcctgaagaa ggaggccttg cggaaccacc 541 agacgccccg gtatgacaat cggtgcagga acatgagcca ggagcaggtg gcccagaagc 601 tggccaagga ccccaagcct gcgatccgct tccgcctgga gcaggtggtg ccagccttcc 661 aggacctggt gtatggctgg aataggcatg aagtggccag cgtggaggga gacccagtca 721 tcatgaagag cgacggcttc cccacatacc acctggcctg cgtggtggac gaccaccaca 781 tgggcatcag ccacgtgctg cgaggctctg agtggctcgt ctccactgcc aagcacctgc 841 tcctctacca ggccctgggc tggcagccac cccacttcgc ccacctgccc ctgctcctca 901 acagggatgg cagcaagctc tccaagaggc aaggggacgt tttcctggag cactttgctg 961 ctgatggctt cctgcccgat tccttgttgg acatcatcac caactgtggc tcaggttttg 1021 cagagaacca aatgggcagg accctgccgg agctgatcac acagttcaac ctgacacagg 1081 tcacctgtca ctcagccctg ctggacctgg agaagctccc agaattcaac agactgcacc 1141 tccagcggct ggtgagcaat gagagccaga ggcgccagct ggtggggaag ctgcaggTCC 1201 TTGTGGAGGA GGCCTTTGGT TGCCAGCTGC AAAACAGGGA TGTCCTCAAC CCAGTCTACG 1261 TGGAGAGGAT CCTCCTGCTG AGACAGGGTC ACATTTGCCG CCTGCAGGAC TTGGTGTCCC 1321 CAGTATACTC TTACCTGTGG ACTCGCCCTG CAGTAGGTCG AGCACAGCTG GACGCCATCT 1381 CGGAGAAGGT GGATGTGATT GCCAAGCGTG TGCTGGGGCT TCTAGAAAGA TCTGGTATGA 1441 GCTTAACTCA GGATATGCTG AATGGAGAAC TGAAGAAGCT ATCAGAAGGT CTGGAAGGCA 1501 CCAAGTACAG TAATGTGATG AAACTCCTTC GGATGGCCCT CAGTGGACAG CAGGTGAGGC 1561 AGGGACACGG GTTGGATTGT TCCCTGGAGC CCCTCATTGA TCCTTTGAAC TTACATTTCC 1621 TGGCAGGCAC TGAGCTCAAT ATTGAGTATA CAAAGGTGAA TGAAACATGC CCAACTTTCT 1681 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1741 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1801 GTGGAAAGGA CGACCCCTGC TGTAAGTCTG TGCTCACACG CGTTAAGTCg acaatcaacc 1861 tctggattac aaaatttgtg aaagatt