Construct: ORF TRCN0000474241
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008393.1_s317c1
- Derived from:
- ccsbBroadEn_13906
- DNA Barcode:
- ACCGATGTATTTAAGCAACTGCCA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYL1 (4632)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474241
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4632 | MYL1 | myosin light chain 1 | NM_079422.3 | 99.7% | 98% | 444delG |
| 2 | human | 4632 | MYL1 | myosin light chain 1 | NM_079420.3 | 75.7% | 72.6% | (many diffs) |
| 3 | mouse | 17901 | Myl1 | myosin, light polypeptide 1 | NM_001113387.1 | 88.8% | 92.6% | (many diffs) |
| 4 | mouse | 17901 | Myl1 | myosin, light polypeptide 1 | XM_011238451.2 | 80.3% | 83.2% | (many diffs) |
| 5 | mouse | 17901 | Myl1 | myosin, light polypeptide 1 | NM_021285.3 | 68.3% | 70.7% | (many diffs) |
| 6 | mouse | 17901 | Myl1 | myosin, light polypeptide 1 | XM_006495763.1 | 68.3% | 70.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 516
- ORF length:
- 450
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cttcagtgct gaccagattg ctgaattcaa ggaggcattt ctcctgtttg 121 acagaacagg tgattcCAAG ATCACCTTAA GCCAGGTCGG TGATGTCCTT CGAGCTCTGG 181 GCACAAATCC CACCAATGCA GAGGTCAGGA AAGTTCTGGG AAACCCCAGC AATGAAGAGC 241 TGAATGCCAA GAAAATTGAG TTTGAACAAT TTCTGCCTAT GATGCAAGCC ATTTCCAACA 301 ACAAGGACCA GGCCACCTAT GAAGACTTTG TTGAGGGTCT GCGTGTCTTT GACAAGGAAG 361 GCAATGGCAC AGTCATGGGT GCTGAACTCC GCCATGTTCT AGCCACCCTG GGTGAAAAGA 421 TGAAAGAGGA AGAAGTGGAA GCCCTGATGG CAGGTCAAGA AGACTCCAAT GGCTGCATCA 481 ACTACGAAGC TTTTGTCAAG CACATCATTC TATCTGCCCA ACTTTCTTGT ACAAAGTGGT 541 TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC 601 TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA 661 ACCGATGTAT TTAAGCAACT GCCAACGCGT TAAGTCgaca atcaacctct ggattacaaa 721 atttgtgaaa gatt