Construct: ORF TRCN0000474242
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006114.1_s317c1
- Derived from:
- ccsbBroadEn_14188
- DNA Barcode:
- TGCCGCCATTGTCGAGCCAGGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF220 (55182)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474242
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55182 | RNF220 | ring finger protein 220 | XR_001737278.2 | 11% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 402
- ORF length:
- 336
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tacaatgttg tgtataaatg ggacaactcc tcgccctcta cctgtcccct 121 ccccctttgg ttgtatgatt ttcTTCTTTT TTAAGAACCC CTGGAAGCAG TGCCTCCTTC 181 AGGGTTGGCT GGGAGCTCGG CCCATCCACC TCTTGGGGTA TCTGCCTCTC TCTCTCCTGT 241 GGTGTCCCTT CCCTCTCCCA TGTGCTCGGT GTTCAGTGGT GTATATTTCT TCTCCCAGAC 301 ATGGGGCACA CGCCCCAAGG GACATGATCC TCTCCTTAGT CTTAGCTCAT GGGGCTCTTT 361 ATAAGGAGTT GGGGGGTAGA GGCAGGAAAT TGGAACCGAG CTGCCCAACT TTCTTGTACA 421 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 481 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 541 AGGACGATGC CGCCATTGTC GAGCCAGGAC AACGCGTTAA GTCgacaatc aacctctgga 601 ttacaaaatt tgtgaaagat t