Construct: ORF TRCN0000474245
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014676.1_s317c1
- Derived from:
- ccsbBroadEn_15497
- DNA Barcode:
- GGCATTTGCTGTTCAGCACCGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MCM2 (4171)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474245
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4171 | MCM2 | minichromosome maintenance ... | NM_004526.4 | 99.9% | 99.8% | 504C>T;1501G>A |
| 2 | human | 4171 | MCM2 | minichromosome maintenance ... | XM_024453531.1 | 98.9% | 98.8% | 0_1ins27;477C>T;1474G>A |
| 3 | human | 4171 | MCM2 | minichromosome maintenance ... | NR_073375.2 | 78.4% | (many diffs) | |
| 4 | mouse | 17216 | Mcm2 | minichromosome maintenance ... | NM_008564.2 | 87.5% | 95.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2778
- ORF length:
- 2712
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggaatcatcg gaatccttca ccatggcatc cagcccggcc cagcgtcggc 121 gaggcaatga tcctctcacc tccagccctg gccgaagctc ccggcgtact gatgccctca 181 cctccagccc tggccgtgac cttccaccat ttgaggatga gtccgagggg ctcctaggca 241 cagaggggcc cctggaggaa gaagaggatg gagaggagct cattggagat ggcatggaaa 301 gggactaccg cgccatccca gagctggacg cctatgaggc cgagggactg gctctggatg 361 atgaggacgt agaggagctg acggccagtc agagggaggc agcagagcgg gccatgcggc 421 agcgtgaccg ggaggctggc cggggcctgg gccgcatgcg ccgtgggctc ctgtatgaca 481 gcgatgagga ggacgaggag cgccctgccc gcaagcgccg ccaggtggag cgggccacgg 541 aggacggcga ggaggacgag gagatgattg agagcatcga gaacctggag gatctcaaag 601 gccactctgt gcgcgagtgg gtgagcatgg cgggcccccg gctggagatc caccaccgct 661 tcaagaactt cctgcgcact cacgtcgaca gccacggcca caacgtcttc aaggagcgca 721 tcagcgacat gtgcaaagag aaccgtgaga gcctggtggt gaactatgag gacttggcag 781 ccagggagca cgtgctggcc tacttcctgc ctgaggcacc ggcggagctg ctgcagatct 841 ttgatgaggc tgccctggag gtggtactgg ccatgtaccc caagtacgac cgcatcacca 901 accacatcca tgtccgcatc tcccacctgc ctctggtgga ggagctgcgc tcgctgaggc 961 agctgcatct gaaccagctg atccgcacca gtggggtggt gaccagctgc actggcgtcc 1021 tgccccagct cagcatggtc aagtacaact gcaacaagtg caatttcgtc ctgggtcctt 1081 tctgccagtc ccagaaccag gaggtgaaac caggctcctg tcctgagtgc cagtcggccg 1141 gcccctttga ggtcaacatg gaggagacca tctatcagaa ctaccagcgt atccgaatcc 1201 aggagagtcc aggcaaagtg gcggctggcc ggctgccccg ctccaaggac gccattctcc 1261 tcgcagatct ggtggacagc tgcaagccag gagacgagat agagctgact ggcatctatc 1321 acaacaacta tgatggctcc ctcaacactg ccaatggctt ccctgtcttt gccactgtca 1381 tcctagccaa ccacgtggcc aagaaggaca acaaggttgc tgtaggggaa ctgaccgatg 1441 aagatgtgaa gatgatcact agcctctcca aggatcagca gatcggagag aagatctttg 1501 ccagcattgc tccttccatc tatggtcatg aagacatcaa gagaggcctg gctctggccc 1561 tgttcagagg ggagcccaaa aacccaggtg gcaagcacaa ggtacgtggt gatatcaacg 1621 tgctcttgtg cggagaccct ggcacagcga agtcgcagtt tctcaagtat attgagaaag 1681 tgtccagccg agccatcttc accactggcc agggggcgtc ggctgtgggc ctcacggcgt 1741 atgtccagcg gcaccctgtc agcagggagt ggaccttgga ggctggggcc ctggttctgg 1801 ctgaccgagg agtgtgtctc attgatgaat ttgacaagat gaatgaccag gacagaacca 1861 gcatccatga ggccatggag caacagagca tctccatctc gaaggctggc atcgtcacct 1921 ccctgcaggc tcgctgcacg gtcattgctg ccgccaaccc cataggaggg cgctacgacc 1981 cctcgctgac tttctctgag aacgtggacc tcacagagcc catcatctca cgctttgaca 2041 tcctgtgtgt ggtgagggac accgtggacc cagtccagga cgagatgctg gcccgcttcg 2101 tggtgggcag ccacgtcaga caccacccca gcaacaagga ggaggagggg ctggccaatg 2161 gcagcgctgc tgagcccgcc atgcccaaca cgtatggcgt ggagcccctg ccccaggagg 2221 tcctgaagaa gtacatcatc tacgccaagg agagggtcca cccgaagctc aaccagatgg 2281 accaggacaa ggtggccaag atgtacagtg acctgaggaa agaatctatg gcgacaggca 2341 gcatccccat tacggtgcgg cacatcgagt cCATGATCCG CATGGCGGAG GCCCACGCGC 2401 GCATCCATCT GCGGGACTAT GTGATCGAAG ACGACGTCAA CATGGCCATC CGCGTGATGC 2461 TGGAGAGCTT CATAGACACA CAGAAGTTCA GCGTCATGCG CAGCATGCGC AAGACTTTTG 2521 CCCGCTACCT TTCATTCCGG CGTGACAACA ATGAGCTGTT GCTCTTCATA CTGAAGCAGT 2581 TAGTGGCAGA GCAGGTGACA TATCAGCGCA ACCGCTTTGG GGCCCAGCAG GACACTATTG 2641 AGGTCCCTGA GAAGGACTTG GTGGATAAGG CTCGTCAGAT CAACATCCAC AACCTCTCTG 2701 CATTTTATGA CAGTGAGCTC TTCAGGATGA ACAAGTTCAG CCACGACCTG AAAAGGAAAA 2761 TGATCCTGCA GCAGTTCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2821 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2881 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGCATTT GCTGTTCAGC 2941 ACCGCGGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt