Construct: ORF TRCN0000474281
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007826.1_s317c1
- Derived from:
- ccsbBroadEn_00864
- DNA Barcode:
- TAAACGGGATTTCTCTTTAAGTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNFRSF9 (3604)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474281
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3604 | TNFRSF9 | TNF receptor superfamily me... | NM_001561.6 | 100% | 100% | |
| 2 | human | 3604 | TNFRSF9 | TNF receptor superfamily me... | XM_006710618.3 | 90% | 89.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 831
- ORF length:
- 765
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg aaacagctgt tacaacatag tagccactct gttgctggtc ctcaactttg 121 agaggacaag atcattgcag gatccttgta gtaactgccc agctggtaca ttctgtgata 181 ataacaggaa tcagatttgc agtccctgtc ctccaaatag tttctccagc gcaggtggac 241 aaaggacctG TGACATATGC AGGCAGTGTA AAGGTGTTTT CAGGACCAGG AAGGAGTGTT 301 CCTCCACCAG CAATGCAGAG TGTGACTGCA CTCCAGGGTT TCACTGCCTG GGGGCAGGAT 361 GCAGCATGTG TGAACAGGAT TGTAAACAAG GTCAAGAACT GACAAAAAAA GGTTGTAAAG 421 ACTGTTGCTT TGGGACATTT AACGATCAGA AACGTGGCAT CTGTCGACCC TGGACAAACT 481 GTTCTTTGGA TGGAAAGTCT GTGCTTGTGA ATGGGACGAA GGAGAGGGAC GTGGTCTGTG 541 GACCATCTCC AGCCGACCTC TCTCCGGGAG CATCCTCTGT GACCCCGCCT GCCCCTGCGA 601 GAGAGCCAGG ACACTCTCCG CAGATCATCT CCTTCTTTCT TGCGCTGACG TCGACTGCGT 661 TGCTCTTCCT GCTGTTCTTC CTCACGCTCC GTTTCTCTGT TGTTAAACGG GGCAGAAAGA 721 AACTCCTGTA TATATTCAAA CAACCATTTA TGAGACCAGT ACAAACTACT CAAGAGGAAG 781 ATGGCTGTAG CTGCCGATTT CCAGAAGAAG AAGAAGGAGG ATGTGAACTG TGCCCAACTT 841 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 901 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 961 CTTGTGGAAA GGACGATAAA CGGGATTTCT CTTTAAGTCC ACGCGTTAAG TCgacaatca 1021 acctctggat tacaaaattt gtgaaagatt