Construct: ORF TRCN0000474350
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006540.1_s317c1
- Derived from:
- ccsbBroadEn_00041
- DNA Barcode:
- AACATTCCGACCATCCTACCGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NR0B1 (190)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474350
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 190 | NR0B1 | nuclear receptor subfamily ... | NM_000475.5 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1476
- ORF length:
- 1410
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggtatggc gggcgagaac caccagtggc agggcagcat cctctacaac atgcttatga 121 gcgcgaagca aacgcgcgcg gctcctgagg ctccagagac gcggctggtg gatcagtgct 181 ggggctgttc gtgcggcgat gagcccgggg tgggcagaga ggggctgctg ggcgggcgga 241 acgtggcgct cctgtaccgc tgctgctttt gcggtaaaga ccacccacgg cagggcagca 301 tcctctacag catgctgacg agcgcaaagc aaacgtacgc ggcaccgaag gcgcccgagg 361 cgacgctggg tccgtgctgg ggctgttcgt gcggctctga tcccggggtg ggcagagcgg 421 ggcttccggg tgggcggccc gtggcactcc tgtaccgctg ctgcttttgt ggtgaagacc 481 acccgcggca gggcagcatc ctctacagct tgctcactag ctcaaagcaa acgcacgtgg 541 ctccggcagc gcccgaggca cggccagggg gcgcgtggtg ggaccgctcc tacttcgcgc 601 agaggccagg gggtaaagag gcgctaccag gcgggcgggc cacggcgctt ctgtaccgct 661 gctgcttttg cggtgaagac cacccgcagc agggcagcac cctctactgc gtgcccacga 721 gcacaaatca agcgcaggcg gctccggagg agcggccgag ggccccctgg tgggacacct 781 cctctggtgc gctgcggccg gtggcgctca agagtccaca ggtggtctgc gaggcagcct 841 cagcgggcct gttgaagacg ctgcgcttcg tcaagtactt gccctgcttc caggtgctgc 901 ccctggacca gcagctggtg ctggtgcgca actgctgggc gtccctgctc atgcttgagc 961 tggcccagga ccgcttgcag ttcgagactg tggaagtctc ggagcccagc atgctgcaga 1021 agatcctcac caccaggcgg cgggagaccg ggggcaacga gccactgccc gtgcccacgc 1081 tgcagcacca tttggcaccg ccggcggagg ccaggaaggt gcccTCCGCC TCCCAGGTCC 1141 AAGCCATCAA GTGCTTTCTT TCCAAATGCT GGAGTCTGAA CATCAGTACC AAGGAGTACG 1201 CCTACCTCAA GGGGACCGTG CTCTTTAACC CGGACGTGCC GGGCCTGCAG TGCGTGAAGT 1261 ACATTCAGGG ACTCCAGTGG GGAACTCAGC AAATACTCAG TGAACACACC AGGATGACGC 1321 ACCAAGGGCC CCATGACAGA TTCATCGAAC TTAATAGTAC CCTTTTCCTG CTGAGATTCA 1381 TCAATGCCAA TGTCATTGCT GAACTGTTCT TCAGGCCCAT CATCGGCACA GTCAGCATGG 1441 ATGATATGAT GCTGGAAATG CTCTGTACAA AGATATACCC AACTTTCTTG TACAAAGTGG 1501 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1561 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1621 AAACATTCCG ACCATCCTAC CGGAAACGCG TTAAGTCgac aatcaacctc tggattacaa 1681 aatttgtgaa agatt