Construct: ORF TRCN0000474372
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013047.1_s317c1
- Derived from:
- ccsbBroadEn_12048
- DNA Barcode:
- GAACAAATAACCGGCCTTTTGATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RHOF (54509)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474372
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54509 | RHOF | ras homolog family member F... | NM_019034.3 | 76.8% | 75.3% | (many diffs) |
| 2 | mouse | 23912 | Rhof | ras homolog family member F... | NM_175092.3 | 68.8% | 66.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 576
- ORF length:
- 510
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGA TGCCCCCGGG GCCCTGGCCC AGACCGCCGC CCCCGGTCCG GGCAGGAAGG 121 AGCTGAAGAT CGTGATCGTG GGCGACGGCG GCTGCGGCAA GACCTCGCTG CTCATGGTGT 181 ACAGCCAGGG CTCCTTCCCC GAGCACTACG CCCCATCGGT GTTCGAGAAG TACACGGCCA 241 GCGTGACCGT TGGCAGCAAG GAGGTGACCC TGAACCTCTA CGACACGGCC GGGCAAGAAG 301 ACTATGACCG GCTGCGGCCC CTGTCCTACC AGAACACCCA CCTCGTGCTC ATCTGCTATG 361 ACGTCATGAA TCCCACCAGC TACGACAACG TCCTCATCAA GTGGTTCCCT GAGGTCACGC 421 ATTTCTGCCG CGGGATCCCC ATGGTGCTCA TCGGCTGCAA GACAGACCTG AGGAAGGACA 481 AGGAGCAGCT GCGGAAGCTC CGGGCCGCCC AGCTGGAGCC CATCACCTAC ATGCAGGTGG 541 GCCGGGGCCA AGACCCTGGG GCACAGCCGT GGCTGTCCCC AACTTTCTTG TACAAAGTGG 601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 721 AGAACAAATA ACCGGCCTTT TGATAACGCG TTAAGTCgac aatcaacctc tggattacaa 781 aatttgtgaa agatt