Construct: ORF TRCN0000474372

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013047.1_s317c1
Derived from:
ccsbBroadEn_12048
DNA Barcode:
GAACAAATAACCGGCCTTTTGATA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RHOF (54509)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474372
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54509 RHOF ras homolog family member F... NM_019034.3 76.8% 75.3% (many diffs)
2 mouse 23912 Rhof ras homolog family member F... NM_175092.3 68.8% 66.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
576
ORF length:
510
Sequence:
1tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG
61TTGGCATGGA TGCCCCCGGG GCCCTGGCCC AGACCGCCGC CCCCGGTCCG GGCAGGAAGG
121AGCTGAAGAT CGTGATCGTG GGCGACGGCG GCTGCGGCAA GACCTCGCTG CTCATGGTGT
181ACAGCCAGGG CTCCTTCCCC GAGCACTACG CCCCATCGGT GTTCGAGAAG TACACGGCCA
241GCGTGACCGT TGGCAGCAAG GAGGTGACCC TGAACCTCTA CGACACGGCC GGGCAAGAAG
301ACTATGACCG GCTGCGGCCC CTGTCCTACC AGAACACCCA CCTCGTGCTC ATCTGCTATG
361ACGTCATGAA TCCCACCAGC TACGACAACG TCCTCATCAA GTGGTTCCCT GAGGTCACGC
421ATTTCTGCCG CGGGATCCCC ATGGTGCTCA TCGGCTGCAA GACAGACCTG AGGAAGGACA
481AGGAGCAGCT GCGGAAGCTC CGGGCCGCCC AGCTGGAGCC CATCACCTAC ATGCAGGTGG
541GCCGGGGCCA AGACCCTGGG GCACAGCCGT GGCTGTCCCC AACTTTCTTG TACAAAGTGG
601TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
661CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
721AGAACAAATA ACCGGCCTTT TGATAACGCG TTAAGTCgac aatcaacctc tggattacaa
781aatttgtgaa agatt

Download FASTA (ORF) (Full)