Construct: ORF TRCN0000474399
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010550.1_s317c1
- Derived from:
- ccsbBroadEn_16102
- DNA Barcode:
- AAGAGTTTCCAAAGATCGGGCATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF418 (147686)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474399
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 147686 | ZNF418 | zinc finger protein 418 | NM_001317029.2 | 99.9% | 100% | 243C>T |
| 2 | human | 147686 | ZNF418 | zinc finger protein 418 | NM_133460.3 | 99.9% | 100% | 243C>T |
| 3 | human | 147686 | ZNF418 | zinc finger protein 418 | XM_017026312.1 | 99.9% | 100% | 243C>T |
| 4 | human | 147686 | ZNF418 | zinc finger protein 418 | XM_017026313.1 | 99.9% | 100% | 243C>T |
| 5 | human | 147686 | ZNF418 | zinc finger protein 418 | NM_001317028.2 | 99.8% | 99.7% | 3_5delGCA;246C>T |
| 6 | human | 147686 | ZNF418 | zinc finger protein 418 | XM_017026309.1 | 99.8% | 99.7% | 3_5delGCA;246C>T |
| 7 | human | 147686 | ZNF418 | zinc finger protein 418 | XM_017026310.1 | 99.8% | 99.7% | 3_5delGCA;246C>T |
| 8 | human | 147686 | ZNF418 | zinc finger protein 418 | XM_017026311.1 | 99.8% | 99.7% | 3_5delGCA;246C>T |
| 9 | human | 147686 | ZNF418 | zinc finger protein 418 | XM_017026308.2 | 98.3% | 98.3% | 1_31del;32_33insT;273C>T |
| 10 | human | 147686 | ZNF418 | zinc finger protein 418 | XM_017026306.2 | 96.9% | 96.8% | (many diffs) |
| 11 | human | 147686 | ZNF418 | zinc finger protein 418 | NM_001317027.2 | 96.8% | 96.8% | 1_64delinsA;306C>T |
| 12 | human | 147686 | ZNF418 | zinc finger protein 418 | XM_017026304.2 | 96.8% | 96.8% | 1_64delinsA;306C>T |
| 13 | human | 147686 | ZNF418 | zinc finger protein 418 | XM_017026305.2 | 96.8% | 96.8% | 1_64delinsA;306C>T |
| 14 | human | 147686 | ZNF418 | zinc finger protein 418 | XM_017026314.2 | 95% | 95.1% | 0_1ins99;144C>T |
| 15 | human | 147686 | ZNF418 | zinc finger protein 418 | NM_001317030.2 | 87.4% | 87.4% | 0_1ins255 |
| 16 | human | 100293516 | ZNF587B | zinc finger protein 587B | NM_001204818.2 | 48.6% | 38.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2097
- ORF length:
- 2028
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagggcact gtggcatttg aagatgtggc tgtgaacttt tcccaggagg 121 agtggagtct ccttagtgag gttcagagat gcctttacca tgacgtgatg ctggagaact 181 gggtacttat atcctccctg ggttgttggt gtggatcaga agatgaggag gcaccttcta 241 agaagagcat ttctatacaa agagtgtctc aggtcagcac tcctggggca ggtgtgtctc 301 ccaagaaggc tcactcttgt gaaatgtgtg gcgcgatctt gggagacatt ttgcacttgg 361 cagatcatca ggggacacat cacaagcaga aactgcacag gtgtgaggca tgggggaata 421 aattgtatga tagttcaaac cgtccgcacc agaatcagta ccttggagag aaaccctata 481 gaagcagtgt tgaggaagca ttgtttgtga agaggtgtaa gttccatgtg tcagaggagt 541 catctatctt cattcagagt ggaaaggact ttttgcccag ctcaggatta ctgctgcagg 601 aggccactca cactggggag aagtcaaaca gcaaacctga gtgtgagtct ccctttcagt 661 ggggagatac tcattacagc tgtggagaat gcatgaaaca ttctagcacc aaacacgtat 721 ttgttcaaca gcagagactt ccctctagag aggaatgtta ttgctgggaa tgtgggaaat 781 cctttagcaa atatgatagc gtcagtaatc atcagagagt tcacactggg aaaagacctt 841 atgaatgtgg agaatgtggg aaatctttta gtcataaggg cagccttgtt cagcatcagc 901 gagttcacac tgggaaaaga ccttatgaat gtggagaatg tgggaaatct tttagtcata 961 agggcagcct tgttcagcat cagcgagttc atactggaga aagaccttat gagtgtggag 1021 aatgtgggaa atcttttagt caaaatggta ctctcattaa acatcaacga gttcacactg 1081 gagaaagacc ttatgagtgt gaagaatgtg ggaaatgttt tactcagaag ggcaatctca 1141 ttcaacatca acgaggtcac actagtgaaa gaccttatga gtgtgaagaa tgtggaaaat 1201 gttttagtca aaagggcacc ctaactgaac atcatcgagt tcacactaga gaacgacctt 1261 atgagtgtgg agaatgtggg aaatctttta gtcgaaaggg acaccttagg aaccatcagc 1321 gaggtcacac tggagaaaga ccttacgagt gtggagaatg tgggaaatct tttagtcgaa 1381 agggcaacct cattcagcat cagcgaagcc acactggaga aaggccttat gagtgtagag 1441 agtgtaggaa attatttagg ggcaagtccc acctcattga acaccagaga gttcacactg 1501 gagaaaggcc atatgaatgt aatgaatgtg ggaaatcatt tcaagacagc tctgggtttc 1561 gtgttcatca gagagttcac actggagaaa aaccgtttga gtgtagtgaa tgtgggaagt 1621 catttcctca aagctgttcc ctccttcgac atcggagagt tcatactgga gaaaggcctt 1681 atgaatgtgg agaatgtgga aagtcatttc atcagagctc ttccctccTT CGACATCAGA 1741 AAACTCACAC TGCAGAAAGA CCTTATGAGT GCAGAGAATG TGGGAAATTC TTCTCCAGTC 1801 TCCTTGAACA CAGGAGAGTT CACACTGGAG AAAGGCCTTA TGAATGCAGG GAATGTGGAA 1861 AAACATTTAC TCGAAGGTCT GCGCATTTTA AACATCAGAG ACTTCATACT CGAGGAAAGC 1921 CTTACGAGTG CAGCGAATGT GGGAAATCCT TTGCTGAAAC CTTCAGTCTT ACTGAACACA 1981 GGAGAGTACA CACTGGAGAA AGGCCTTATG AGTGCAGTGA ATGTGGAAAA TCATTTCATC 2041 GAAGCTCTTC TCTCCTTCGA CATCAGAGAG TTCACACAGA AAGAAGTCCT TACAAGTTGC 2101 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2161 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2221 ATATATCTTG TGGAAAGGAC GAAAGAGTTT CCAAAGATCG GGCATTACGC GTTAAGTCga 2281 caatcaacct ctggattaca aaatttgtga aagatt