Construct: ORF TRCN0000474415
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001671.1_s317c1
- Derived from:
- ccsbBroadEn_02451
- DNA Barcode:
- GGCCCCAGTGCCCGGGTTCGTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOXB13 (10481)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474415
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10481 | HOXB13 | homeobox B13 | NM_006361.6 | 100% | 100% | |
| 2 | mouse | 15408 | Hoxb13 | homeobox B13 | NM_008267.3 | 87% | 93% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 918
- ORF length:
- 852
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcccggcaat tatgccacct tggatggagc caaggatatc gaaggcttgc 121 tgggagcggg aggggggcgg aatctggtcg cccactcccc tctgaccagc cacccagcgg 181 cgcctacgct gatgcctgct gtcaactatg cccccttgga tctgccaggc tcggcggagc 241 cgccaaagca atgccaccca tgccctgggg tgccccaggg gacgtcccca gctcccgtgc 301 cttatggtta ctttggaggc gggtactact cctgccgagt gtcccggagc tcgctgaaac 361 cctgtgccca ggcagccacc ctggccgcgt accccgcgga gactcccacg gccggggaag 421 agtaccccag ccgccccact gagtttgcct tctatccggg ataTCCGGGA ACCTACCAGC 481 CTATGGCCAG TTACCTGGAC GTGTCTGTGG TGCAGACTCT GGGTGCTCCT GGAGAACCGC 541 GACATGACTC CCTGTTGCCT GTGGACAGTT ACCAGTCTTG GGCTCTCGCT GGTGGCTGGA 601 ACAGCCAGAT GTGTTGCCAG GGAGAACAGA ACCCACCAGG TCCCTTTTGG AAGGCAGCAT 661 TTGCAGACTC CAGCGGGCAG CACCCTCCTG ACGCCTGCGC CTTTCGTCGC GGCCGCAAGA 721 AACGCATTCC GTACAGCAAG GGGCAGTTGC GGGAGCTGGA GCGGGAGTAT GCGGCTAACA 781 AGTTCATCAC CAAGGACAAG AGGCGCAAGA TCTCGGCAGC CACCAGCCTC TCGGAGCGCC 841 AGATTACCAT CTGGTTTCAG AACCGCCGGG TCAAAGAGAA GAAGGTTCTC GCCAAGGTGA 901 AGAACAGCGC TACCCCTTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 961 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1021 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGCCCCA GTGCCCGGGT 1081 TCGTTCAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt