Construct: ORF TRCN0000474431
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009806.1_s317c1
- Derived from:
- ccsbBroadEn_09372
- DNA Barcode:
- TTCCGATCGCGATCTCCTCGCACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERI2 (112479)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474431
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112479 | ERI2 | ERI1 exoribonuclease family... | NM_080663.3 | 99.7% | 99.6% | 504G>A;802C>A |
| 2 | human | 112479 | ERI2 | ERI1 exoribonuclease family... | NM_001142725.2 | 43.3% | 37.9% | (many diffs) |
| 3 | mouse | 71151 | Eri2 | exoribonuclease 2 | XM_006508210.3 | 64.5% | 65.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1050
- ORF length:
- 984
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gaccaagcgg ctcgcgcggc agcttggatt aattaggaga aagtcaattg 121 cgccagcaaa tggaaatctc ggaagaagca aatccaagca gttgtttgac tacttaattg 181 tcattgattt tgaatcgaca tgctggaatg atgggaagca ccaccatagc caggaaataa 241 ttgagtttcc agcagtgttg ctgaacacat caactggaca gattgactct gagttccagg 301 cttatgttca acctcaggaa catccaattc tttcagaatt ttgcatggaa ttgacaggca 361 taaagcaggc tcaagttgat gaaggagtcc ctctgaagat ttgcttatct cagttctgta 421 aatggattca taagattcag caacagaaga acattatttt tgctactggg atttcagagc 481 cttctgcttc tgaagtaaaa ttatgtgcat ttgttacttg gtcagactgg gacttggggg 541 tttgcctgga gtatgagtgt aaaagaaaac agctgttaaa acctgtgttt ttaaattctt 601 ggattgatct cagagcaact taCAAGCTTT TCTATAGGAG AAAACCAAAA GGACTAAGTG 661 GTGCCTTGCA GGAAGTAGGA ATAGAATTCT CAGGACGAGA ACATTCTGGG TTGGACGATT 721 CTCGGAATAC TGCCCTTCTT GCTTGGAAAA TGATCAGAGA TGGTTGTGTA ATGAAAATTA 781 CAAGGTCGTT GAACAAGGGT CCCTTCCTCT TGCCTTCGTG GACCTGGAAC AGTGATCTGG 841 CCTCAGGGGA CCAGCATGCT TTTCTTAAGC AAGAATTTGG CTGTGGAACC TACAGAACCT 901 TACTTCAGAA GCCCAATATG AGTAAACAGG AAAAGGGGAA TATTCTCTGG TTGACAATGG 961 TGTGGCTGTC ACTGGCATGT CTGCAAAGGA AAAACTACAA TGACTGCATG TTAAACACAG 1021 CATCACAGAC TGTTACCACT GAAAAATTTT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1081 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1141 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTCCG 1201 ATCGCGATCT CCTCGCACGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1261 tgaaagatt