Construct: ORF TRCN0000474461
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015100.1_s317c1
- Derived from:
- ccsbBroadEn_11154
- DNA Barcode:
- TCTTTGCCCTTGTCGTGAGTCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPRR1B (6699)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474461
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6699 | SPRR1B | small proline rich protein 1B | NM_003125.3 | 98.8% | 98.8% | 27T>C;32C>T;162C>T |
| 2 | human | 6698 | SPRR1A | small proline rich protein 1A | NM_001199828.2 | 95.8% | 94.3% | (many diffs) |
| 3 | human | 6698 | SPRR1A | small proline rich protein 1A | NM_005987.4 | 95.8% | 94.3% | (many diffs) |
| 4 | mouse | 20753 | Sprr1a | small proline-rich protein 1A | NM_009264.2 | 54.6% | 54.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 240
- ORF end:
- 507
- ORF length:
- 267
- Sequence:
-
1 GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA TGCGATGGAG TTTCCCCACA CTGAGTGGGT 61 GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC TCCTTGGAAT TTGCCCTTTT 121 TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG GTTCAAAGTT TTTTTCTTCC 181 ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG TTTGTACAAA AAAGTTGGCA 241 TGAGTTCCCA GCAGCAGAAG CAGCCCTGCA TCCCACCCCC TCAGCTTCAG CAGCAGCAGG 301 TGAAACAGCC TTGCCAGCCT CCACCTCAGG AACCATGCAT CCCCAAAACC AAGGAGCCCT 361 GCCACCCCAA GGTGCCTGAG CCCTGCCACC CCAAAGTGCC TGAGCCCTGC CAGCCCAAGG 421 TTCCAGAGCC ATGCCACCCC AAGGTGCCTG AGCCCTGCCC TTCAATAGTC ACTCCAGCAC 481 CAGCCCAGCA GAAGACCAAG CAGAAGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 541 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 601 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCTTTGCC 661 CTTGTCGTGA GTCTTAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 721 aagatt