Construct: ORF TRCN0000474463

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002446.1_s317c1
Derived from:
ccsbBroadEn_09717
DNA Barcode:
CCTATGAGGTGGCTTTCATAAAAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NXNL2 (158046)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474463
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 158046 NXNL2 nucleoredoxin like 2 NM_145283.3 99.7% 99.2% 227C>G
2 human 158046 NXNL2 nucleoredoxin like 2 XM_005251727.3 81% 68.3% (many diffs)
3 human 158046 NXNL2 nucleoredoxin like 2 NM_001161625.1 77.3% 59.7% (many diffs)
4 human 158046 NXNL2 nucleoredoxin like 2 XM_011518276.2 75.9% 70.7% (many diffs)
5 human 158046 NXNL2 nucleoredoxin like 2 XM_011518275.2 57.7% 51.4% (many diffs)
6 human 158046 NXNL2 nucleoredoxin like 2 XR_001746187.1 39.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
471
ORF length:
405
Sequence:
1tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG
61TTGGTATGGT TGACATTCTG GGCGAGCGGC ACCTGGTGAC CTGTAAGGGC GCGACGGTGG
121AGGCCGAGGC GGCGCTGCAG AACAAGGTGG TGGCACTGTA CTTCGCGGCG GCCCGGTGCG
181CGCCGAGCCG CGACTTCACG CCGCTGCTCT GCGACTTCTA TACGGCGCTG GTGGCCGAGG
241CGCGGCGGCC CGCGCCCTTC GAAGTGGTCT TCGTGTCAGC CGACGGCAGC TGCCAGGAGA
301TGCTGGACTT CATGCGCGAG CTGCATGGCG CCTGGCTGGC GCTGCCCTTC CACGACCCCT
361ACCGGCAACG GAGTCTCGCT CTGTTGCCCA GGCTGGAGTG CAGTGGCGTG ATCTTAGCTC
421ACTGCAACCT TTGCCTCCTG GGTTCAAGTG ATTCTCTAGC CTTAGCCTCC TGCCCAACTT
481TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
541CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
601CTTGTGGAAA GGACGACCTA TGAGGTGGCT TTCATAAAAA ACGCGTTAAG TCgacaatca
661acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)