Construct: ORF TRCN0000474463
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002446.1_s317c1
- Derived from:
- ccsbBroadEn_09717
- DNA Barcode:
- CCTATGAGGTGGCTTTCATAAAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NXNL2 (158046)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474463
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 158046 | NXNL2 | nucleoredoxin like 2 | NM_145283.3 | 99.7% | 99.2% | 227C>G |
| 2 | human | 158046 | NXNL2 | nucleoredoxin like 2 | XM_005251727.3 | 81% | 68.3% | (many diffs) |
| 3 | human | 158046 | NXNL2 | nucleoredoxin like 2 | NM_001161625.1 | 77.3% | 59.7% | (many diffs) |
| 4 | human | 158046 | NXNL2 | nucleoredoxin like 2 | XM_011518276.2 | 75.9% | 70.7% | (many diffs) |
| 5 | human | 158046 | NXNL2 | nucleoredoxin like 2 | XM_011518275.2 | 57.7% | 51.4% | (many diffs) |
| 6 | human | 158046 | NXNL2 | nucleoredoxin like 2 | XR_001746187.1 | 39.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 471
- ORF length:
- 405
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGTATGGT TGACATTCTG GGCGAGCGGC ACCTGGTGAC CTGTAAGGGC GCGACGGTGG 121 AGGCCGAGGC GGCGCTGCAG AACAAGGTGG TGGCACTGTA CTTCGCGGCG GCCCGGTGCG 181 CGCCGAGCCG CGACTTCACG CCGCTGCTCT GCGACTTCTA TACGGCGCTG GTGGCCGAGG 241 CGCGGCGGCC CGCGCCCTTC GAAGTGGTCT TCGTGTCAGC CGACGGCAGC TGCCAGGAGA 301 TGCTGGACTT CATGCGCGAG CTGCATGGCG CCTGGCTGGC GCTGCCCTTC CACGACCCCT 361 ACCGGCAACG GAGTCTCGCT CTGTTGCCCA GGCTGGAGTG CAGTGGCGTG ATCTTAGCTC 421 ACTGCAACCT TTGCCTCCTG GGTTCAAGTG ATTCTCTAGC CTTAGCCTCC TGCCCAACTT 481 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 541 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 601 CTTGTGGAAA GGACGACCTA TGAGGTGGCT TTCATAAAAA ACGCGTTAAG TCgacaatca 661 acctctggat tacaaaattt gtgaaagatt